Abstract
AMP-activated protein kinase (AMPK) represents the mammalian form of the core component of a kinase cascade that is conserved between fungi, plants, and animals. AMPK plays a major role in protecting mammalian cells from metabolic stress by switching off biosynthetic pathways that require ATP and switching on ATP-regenerating pathways. In this report, we describe the isolation and characterization of the gene for the noncatalytic bovine γ1 subunit of AMPK. The bovine ampkγ1 (PRKAG1) gene spans in excess of 14 kb and is located at BTA 5q21–q22. It consists of 12 exons ranging in size from 38 b to 166 b, interspersed with 11 introns that range between 97 b and 6753 b in length. The coding region of the bovine gene shares 93% and 90% nucleotide sequence similarity with its human and rat counterparts, and the bovine AMPKγ1 protein is 98% and 95% identical to its human and rat homologs, respectively, in amino acid sequence. SNP discovery using a cattle DNA panel revealed a number of polymorphisms that may be useful for the evaluation of ampkγ1 as a candidate gene for energy metabolism-related production traits.
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The authors gratefully acknowledge the assistance of Mr. Y. Fong, Agriculture and Agri-Food Canada, Centre for Food and Animal Research, Ottawa, Canada, in the cloning of the bovine ampkγ1 gene.
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Abbreviations:
ampk - gene, cDNA, or mRNA coding for AMPK; AMPK - AMP-activated protein kinase; AMP - adenosine 3′, 5′-monophosphate; ATP - adenosine 3′ 5′-triphosphate; b - base(s); kb - kilobase(s) or 1000 b; CBS - cystathionine β-synthase domain; PCR - polymerase chain reaction; RTase - reverse transcriptase; SNP - single nucleotide polymorphism.
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Benkel, B., Kollers, S., Fries, R. et al. Characterization of the bovine ampkγ1 gene. Mamm Genome 16, 194–200 (2005). https://doi.org/10.1007/s00335-004-2426-9
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DOI: https://doi.org/10.1007/s00335-004-2426-9