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Transcriptional profiling of skeletal muscle tissue from two breeds of cattle

Mammalian Genome volume 16, pages 201–210 (2005)Cite this article

Abstract

We used a 9.6 K cattle muscle/fat cDNA microarray to study gene expression differences between the longuissimus dorsi (LD) muscle of Japanese Black (JB) and Holstein (HOL) cattle. JB cattle exhibit an unusual ability to accumulate intramuscular adipose tissue with fat melting points lower than that in other breeds. The LD biopsies from three JB (Tajima strain) and three HOL animals were used in this breed comparison. Seventeen genes were identified as preferentially expressed in LD samples from JB and seven genes were found to be expressed more highly in HOL. The expression of six selected differentially expressed genes was confirmed by quantitative real-time PCR. The genes more highly expressed in JB are associated with unsaturated fatty acid synthesis, fat deposition, and the thyroid hormone pathway. These results are consistent with the increased amounts and proportions of monounsaturated fatty acids observed in the muscle of JB animals. By discovering as yet uncharacterized genes that are differentially regulated in this comparison, the work may lead us to a better understanding of the regulatory pathways involved in the development of intramuscular adipose tissue.

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Acknowledgments

This research was supported in Australia by the Cooperative Research Center for Cattle and Beef Quality, and we acknowledge the many other resources and opportunities provided to us by the staff of the Center. The authors thank Tony Vuocolo and Siok Hwee Tan for supplying primer sequences and Dr. Akio Oka (Livestock Research Center, Hyogo Prefectural Institute of Agriculture, Forestry and Fisheries, Kasai, Japan) for his contribution to the animal experiments. In Japan, the work was facilitated significantly by the support of Nitchiku Japan, and we thank them for that support.

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Affiliations

  1. Cooperative Research Center for Cattle and Beef Quality, CSIRO Livestock Industries, Queensland Bioscience Precinct, 306 Carmody Rd., St. Lucia, Brisbane, Queensland, 4067, Australia

    Yong-Hong Wang, Keren A. Byrne, Antonio Reverter, Gregory S. Harper, Sean M. McWilliam & Sigrid A. Lehnert

  2. Laboratory of Animal Breeding and Genetics, Faculty of Agriculture, Kobe University, Kobe-shi, 657-8501, Japan

    Masaaki Taniguchi & Hideyuki Mannen

  3. Food Resources Education & Research Center, Kobe University, Kasai, 675-2103, Japan

    Kenji Oyama

Authors
  1. Yong-Hong Wang
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  2. Keren A. Byrne
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  3. Antonio Reverter
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  4. Gregory S. Harper
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  5. Masaaki Taniguchi
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  6. Sean M. McWilliam
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  7. Hideyuki Mannen
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  8. Kenji Oyama
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  9. Sigrid A. Lehnert
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Corresponding author

Correspondence to Yong-Hong Wang.

Additional information

The nucleotide sequence data reported in this article have been submitted to GenBank and have been assigned the Accession numbers CF613981–CF615389.

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Wang, YH., Byrne, K.A., Reverter, A. et al. Transcriptional profiling of skeletal muscle tissue from two breeds of cattle. Mamm Genome 16, 201–210 (2005). https://doi.org/10.1007/s00335-004-2419-8

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  • DOI: https://doi.org/10.1007/s00335-004-2419-8

Keywords

  • Differentially Express
  • Array Element
  • Base Cluster Method
  • Cardiac Ankyrin Repeat Protein
  • Intramuscular Adipose Tissue