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Conservation of genomic imprinting at the XIST, IGF2, and GTL2 loci in the bovine

Mammalian Genome volume 15, pages 966–974 (2004)Cite this article

Abstract

Genomic imprinting is theorized to exist in all placental mammals and some marsupials; however, extensive comparative analysis of animals aside from humans and mice remains incomplete. Here we report conservation of genomic imprinting in the bovine at the X chromosome inactivation–specific transcript (XIST), insulin-like growth factor 2 (IGF2), and gene trap locus 2 (GTL2) loci. Coding single nucleotide polymorphisms (SNPs) between Bos gaurus and Bos taurus were detected at the XIST, IGF2, and GTL2 loci, which have previously been identified as imprinted in either humans, mice, or sheep. Expression patterns of parental alleles in F1 hybrids indicated preferential paternal expression at the XIST locus solely in the chorion of females, whereas analysis of the IGF2 and GTL2 loci indicated preferential paternal and maternal expression of alleles, respectively, in both fetal and placental tissues. Comparative sequence analysis of the XIST locus and adjacent regions suggests that repression of the maternal allele in the bovine is controlled by a different mechanism than in mice, further reinforcing the importance of comparative analysis of imprinting.

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Acknowledgments

We thank Gary Hansen and members of Dr. Piedrahita’s laboratory for assistance with generating and maintenance of the experimental cattle. This research was supported by NIH grant HL51587 and a Texas A&M University, College of Veterinary Medicine Signature grant.

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Authors and Affiliations

  1. Department of Veterinary Anatomy and Public Health, College of Veterinary Medicine, Texas A&M University, College Station, Texas, 77843, USA

    Scott V. Dindot, Kathleen C. Kent & Bret Evers

  2. Center for Conservation and Research, Henry Doorly Zoo, Omaha, Nebraska, 68107, USA

    Naida Loskutoff

  3. Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A&M University, College Station, Texas, 77843, USA

    James Womack

  4. Molecular Biomedical Science, College of Veterinary Medicine, North Carolina State University, 617 Hutton St., Raleigh, North Carolina, 27606, USA

    Jorge A. Piedrahita

Authors
  1. Scott V. Dindot
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  2. Kathleen C. Kent
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  3. Bret Evers
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  4. Naida Loskutoff
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  5. James Womack
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  6. Jorge A. Piedrahita
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Correspondence to Jorge A. Piedrahita.

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Dindot, S.V., Kent, K.C., Evers, B. et al. Conservation of genomic imprinting at the XIST, IGF2, and GTL2 loci in the bovine. Mamm Genome 15, 966–974 (2004). https://doi.org/10.1007/s00335-004-2407-z

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  • DOI: https://doi.org/10.1007/s00335-004-2407-z

Keywords

  • Imprint Gene
  • Comparative Sequence Analysis
  • Allelic Expression
  • Xist Gene
  • Xist Locus