Skip to main content
SpringerLink
Log in

Loci affecting male fertility in hybrids between Mus macedonicus and C57BL/6

  • Published:
Mammalian Genome Aims and scope Submit manuscript

Abstract

Male F1 hybrids between inbred strains and Mus macedonicus have very small testes and are sterile. Cytological analysis of testes shows very few meioses. To determine the genetic basis for this sterility, (C57BL/6J × Mus macedonics) F1 females were mated to males from C57BL/10J. In about half the male progeny no meiosis I was observed. About half of the animals that progressed through meiosis I showed other indications of low fertility and the balance appeared fertile. QTL analysis of the progeny suggested that loci on proximal Chrs 17 and X were involved in the sterility and a locus on Chr X in variation of body weight. There is also evidence that X//Y dissociation of the pseudo-autosomal region occurs. The QTLs on Chrs X and 17 together account for about 37% of the variance for testis weight. Congenic lines B.MAC-X(1-38), and B.MAC-17(1-23) have been constructed using a modified speed congenic approach. Testis tubules from B.MAC-X(1-38) are narrow and vacuolated. They contain only Sertoli cells and mitotically dividing spermatogonia. Very occasionally a meiotic metaphase can be observed, but no sperm are produced. Homozygous males from B.MAC-17(1-23) are sterile, producing sperm heads but no complete sperm.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  • GA Brockmann CS Haley U Renne SA Knott M Schwerin (1998) ArticleTitleQuantitative trait loci affecting body weight and fatness from a mouse line selected for extreme high growth Genetics 150 369–381 Occurrence Handle1:CAS:528:DyaK1cXmtFKgsLc%3D Occurrence Handle9725853

    CAS  PubMed  Google Scholar 

  • TA Dragani ZB Zeng F Canzian M Gariboldi MT Ghilarducci et al. (1995) ArticleTitleMapping of body weight loci on mouse chromosome X Mamm Genome 6 778–781 Occurrence Handle1:CAS:528:DyaK28XktVWlug%3D%3D Occurrence Handle8597632

    CAS  PubMed  Google Scholar 

  • Elliott RW (2003) Mouse genome sequence-based maps for Chrs 17 and X. ftp://ftp.informatics.jax.org/%2Fpub/datasets/index.html

  • RW Elliott DR Miller RS Pearsall C Hohman Y Zhang et al. (2001) ArticleTitleGenetic analysis of testis weight and fertility in an interspecies hybrid congenic strain for Chr X Mamm Genome 12 45–51 Occurrence Handle10.1007/s003350010234 Occurrence Handle1:CAS:528:DC%2BD3MXptlGqug%3D%3D Occurrence Handle11178743

    Article  CAS  PubMed  Google Scholar 

  • J Forejt P Ivanyi (1974) ArticleTitleGenetic studies on male sterility of hybrids between laboratory and wild mice (Mus musculus L.) Genet Res 24 189–206 Occurrence Handle1:STN:280:CSqC3MzkslY%3D Occurrence Handle4452481

    CAS  PubMed  Google Scholar 

  • J Fosella SA Samant LM Silver SM King KT Vaughan et al. (2000) ArticleTitleAn axonemal dynein at the hybrid sterility 6 locus: implications for t-haplotype-specific male sterility and the evolution of species barriers Mamm Genome 11 8–15 Occurrence Handle10.1007/s003350010003 Occurrence Handle10602986

    Article  PubMed  Google Scholar 

  • JL Guenet C Nagamine D Simon–Chazottes X Montagutelli F Bonhomme (1990) ArticleTitleHst−3: an X-linked hybrid sterility gene Genet Res 56 163–165 Occurrence Handle1:STN:280:By6C3cnnsFE%3D Occurrence Handle2272506

    CAS  PubMed  Google Scholar 

  • HT Imai Y Matsuda T Shiroishi K Moriwaki (1981) ArticleTitleHigh frequency for X–Y chromosome dissociation in primary spermatocytes of F1 hybrids between Japanese wild mice (Mus musculus molossinus) and inbred laboratory mice Cytogenet Cell Genet 29 166–175 Occurrence Handle1:STN:280:Bi6C1cjkslQ%3D Occurrence Handle7226897

    CAS  PubMed  Google Scholar 

  • X Liu F Oliver SD Brown P Denny PD Keightley (2001) ArticleTitleHigh-resolution quantitative trait locus mapping for body weight in mice by recombinant progeny testing Genet Res 77 191–197 Occurrence Handle10.1017/S0016672301004943 Occurrence Handle1:CAS:528:DC%2BD3MXktVGqsL4%3D Occurrence Handle11355574

    Article  CAS  PubMed  Google Scholar 

  • KF Manly JM Olson (1999) ArticleTitleOverview of QTL mapping software and introduction to map manager QT Mamm Genome 10 327–334 Occurrence Handle10.1007/s003359900997 Occurrence Handle1:CAS:528:DyaK1MXhvFyqtLY%3D Occurrence Handle10087288

    Article  CAS  PubMed  Google Scholar 

  • Y Matsuda T Hirobe VM Chapman (1991) ArticleTitleGenetic basis of X–Y chromosome dissociation and male sterility in interspecific hybrids Proc Natl Acad Sci USA 88 4850–4854 Occurrence Handle1:STN:280:By6B2s7ivFQ%3D Occurrence Handle2052565

    CAS  PubMed  Google Scholar 

  • SH Pilder MF Hammer LM Silver (1991) ArticleTitleA novel mouse chromosome 17 hybrid sterility locus: implications for the origin of t haplotypes Genetics 129 237–246 Occurrence Handle1:CAS:528:DyaK38XnslOkuw%3D%3D Occurrence Handle1936961

    CAS  PubMed  Google Scholar 

  • SH Pilder P Olds–Clarke DM Phillips LM Silver (1993) ArticleTitleHybrid sterility−6: a mouse t complex locus controlling sperm flagellar assembly and movement Dev Biol 159 631–642 Occurrence Handle10.1006/dbio.1993.1270 Occurrence Handle1:STN:280:ByuD3cbptlw%3D Occurrence Handle8405685

    Article  CAS  PubMed  Google Scholar 

  • SH Pilder P Olds–Clarke JM Orth WF Jester L Dugan (1997) ArticleTitleHst7: A male sterility mutation perturbing sperm motility, flagellar assembly, and mitochondrial sheath differentiation J Androl 18 663–671 Occurrence Handle1:STN:280:DyaK1c%2Fpt1CgtA%3D%3D Occurrence Handle9432139

    CAS  PubMed  Google Scholar 

  • SA Samant J Fossella LE Silver S Pilder (1999) ArticleTitleMapping and cloning recombinant breakpoints demarcating the hybrid sterility 6-specific sperm tail assembly defect Mamm Genome 10 88–94 Occurrence Handle10.1007/s003359900950 Occurrence Handle1:CAS:528:DyaK1MXotVyntw%3D%3D Occurrence Handle9922385

    Article  CAS  PubMed  Google Scholar 

  • Z Trachtulec M Mnukova–Fajdelova RMJ Hamvas S Gregorova WE Mayer et al. (1997) ArticleTitleIsolation of candidate hybrid sterility 1 genes by cDNA selection in a 1.1 megabase pair region on mouse chromosome 17 Mamm Genome 8 312–316 Occurrence Handle10.1007/s003359900430 Occurrence Handle1:CAS:528:DyaK2sXjtVOhsLc%3D Occurrence Handle9107673

    Article  CAS  PubMed  Google Scholar 

  • L Yuan J-G Liu J Zhao E Brundell B Daneholt et al. (2000) ArticleTitleThe murine SCP3 gene is required for synaptonemal complex assembly, chromosome synapsis and male fertility Mol Cell 5 73–83 Occurrence Handle10.1016/S1097-2765(00)80404-9 Occurrence Handle1:CAS:528:DC%2BD3cXpvFKntg%3D%3D Occurrence Handle10678170

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

This study was supported by NIH grant GM33160 to R.W. Elliott. The work was initiated by Verne Chapman. We thank Yoichi Matsuda for providing training in the cytological procedures. This project utilized core facilities partially supported by RPCI’s NCI-funded Cancer Center Support Grant P30 CA016056.

Author information

Authors and Affiliations

  1. Department of Molecular and Cellular Biology, Roswell Park Cancer Institute, Carlton and Elm Sts., Buffalo , New York, , 14263, USA

    Rosemary W. Elliott, Diane Poslinski, Debra Tabaczynski, Colleen Hohman & Joanne Pazik

Authors
  1. Rosemary W. Elliott
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Diane Poslinski
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Debra Tabaczynski
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Colleen Hohman
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Joanne Pazik
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Rosemary W. Elliott.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Elliott, R.W., Poslinski, D., Tabaczynski, D. et al. Loci affecting male fertility in hybrids between Mus macedonicus and C57BL/6. Mamm Genome 15, 704–710 (2004). https://doi.org/10.1007/s00335-004-2388-y

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00335-004-2388-y

Keywords

Search

Navigation