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Bovine β-defensins: Identification and characterization of novel bovine β-defensin genes and their expression in mammarygland tissue

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Abstract

β-Defensin genes code for multifunctional peptides with a broad-range antimicrobial activity. In this project we hypothesized that β-defensin genes may be candidate genes for resistance to mastitis. In this article we describe the identification and genomic characterization of eight bovine β-defensin genes, including six novel defensin genes and two pseudogenes. Expression in the bovine mammary gland of one of the novel genes, DEFB401, has been demonstrated, as well as the expression of LAP, TAP, DEFB1, BNBD3, BNBD9, and BNBD12. For genomic characterization, 20 BACs from two different bovine BAC libraries (RZPD numbers 750 and 754) were isolated by PCR screening with β-defensin consensus primers derived from published sequences. PCR products from BACs generated with consensus primers have been subcloned and sequenced, revealing a total of 16 genes and two pseudogenes. Six novel β-defensin genes share the typical exon–intron structure and are highly homologous to published bovine β-defensin genes. They are named DEFB401–DEFB405 and LAP-like, and two novel pseudogenes are named EBD-P and EBD-P2. Analysis of mammary gland tissue-derived cDNA from nine cows with different clinical findings demonstrated the expression of several β-defensin genes mentioned above. First results indicate that the lactational status of the cow presumably has no influence on gene expression. Competent knowledge of antimicrobial activity of β-defensins from literature, the abundance of β-defensin mRNA in the bovine mammary gland, and the inducibility of some genes give first evidence that β-defensins may play a role in local host defense during udder infections.

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Acknowledgments

This research was carried out with grateful financial support of the German Research Foundation (DFG) and is part of “Sonderforschungsbereich 617.” We thank Stefanie Schultze for technical assistance.

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Authors and Affiliations

  1. Institut für Tierzucht und Tierhaltung, Christian-Albrechts-Universität, D-24098, Kiel, Germany

    Susanne Roosen, Kay Exner, Sven Paul, Ernst Kalm & Christian Looft

  2. Klinik für Dermatologie, Venerologie und Allergologie, Universitätsklinikum Schleswig-Holstein, Campus Kiel, D-24098, Kiel, Germany

    Jens-Michael Schröder

Authors
  1. Susanne Roosen
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  2. Kay Exner
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  3. Sven Paul
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  4. Jens-Michael Schröder
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  5. Ernst Kalm
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  6. Christian Looft
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Corresponding author

Correspondence to Christian Looft.

Additional information

The nucleotide sequence data reported in this article have been submitted to EMBL and have been assigned the accession numbers AJ563279–AJ563283, AJ567353–AJ567365, AJ567990–AJ567993, and AJ620296.

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Roosen, S., Exner, K., Paul, S. et al. Bovine β-defensins: Identification and characterization of novel bovine β-defensin genes and their expression in mammarygland tissue. Mamm Genome 15, 834–842 (2004). https://doi.org/10.1007/s00335-004-2387-z

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  • DOI: https://doi.org/10.1007/s00335-004-2387-z

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