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Analysis of a marsupial MHC region containing two recently duplicated class I loci

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Abstract

A 37-kb cosmid containing two complete major histocompatibility complex (MHC) class I α chain loci from the opossum Monodelphis domestica was isolated, fully sequenced, and characterized. This sequence represents the largest contiguous genomic sequence reported for the MHC region of a nonplacental mammal. Based on particular conserved amino acid residues, and limited expression analyses, the two MHC-I loci, designated ModoUB and ModoUC, appear to encode functional MHC-I molecules. The two coding regions are 98% identical at the nucleotide level; however, their promoter regions differ significantly. Two CpG islands present in the cosmid sequence correspond to the two coding regions. Twelve microsatellites and six retroelements were also present in the cosmid. The retroelements share highest sequence homology to the CORE–SINE family of retroelements. Due to high sequence identity, it is very likely that ModoUB and ModoUC loci are products of recent gene duplication that occurred less than 4 million years ago.

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Acknowledgments

The authors wish to thank G.H. Rosenberg for his assistance in cosmid sequencing strategies, and J. Salazar and D. Burke for valuable technical assistance. This material is based upon work supported by the National Science Foundation under Grant No. 9981960.

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Authors and Affiliations

  1. Department of Biology, The University of New Mexico, Albuquerque, New Mexico, 87131, USA

    Katarzyna B. Miska, April M. Wright, Rachel Lundgren, Robert Sasaki–McClees, Amy Osterman & Robert D. Miller

  2. Department of Cell Biology and Physiology, The University of New Mexico School of Medicine, Albuquerque, New Mexico, 87131, USA

    James M. Gale

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  1. Katarzyna B. Miska
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  2. April M. Wright
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  3. Rachel Lundgren
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  7. Robert D. Miller
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Correspondence to Robert D. Miller.

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Miska, K.B., Wright, A.M., Lundgren, R. et al. Analysis of a marsupial MHC region containing two recently duplicated class I loci. Mamm Genome 15, 851–864 (2004). https://doi.org/10.1007/s00335-004-2224-4

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