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Characterization of Pisrt1/Foxl2 in Ellobius lutescens and exclusion as sex-determining genes

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Abstract

The rodent Ellobius lutescens is an exceptional mammal which determines male sex constitutively without the SRY gene and, therefore, may serve as an animal model for human 46,XX female-to-male sex reversal. It was suggested that other factors of the network of sex-determining genes determine maleness in these animals. However, some sex-determining genes like SOX9 and SF1 have already been excluded by segregation analysis as primary sex-determining factors in E. lutescens. In this work, we have cloned and characterized two genes of the PIS (polled intersex syndrome) gene interval, which were reported as candidates in female-to-male sex reversal in hornless goats recently. The genes Foxl2 and Pisrt1 from that interval were identified in E. lutescens DNA and mapped to Chromosome 8. We have excluded linkage of Foxl2 and Pisrt1 loci with the sex of the animals. Hence, the involvement of this gene region in sex determination may be specific for goats and is not a general mechanism of XX sex reversal or XX male sex determination.

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Acknowledgments

This work was supported by research grant JU 342/7-3 from the Deutsche Forschungsgemeinschaft DFG.

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Authors and Affiliations

  1. Department of Human Genetics, University of Ulm, Albert-Einstein-Allee 11, 89081, Ulm, Germany

    Annette Baumstark, Horst Hameister & Walter Just

  2. Department of Virology, The National Veterinary Institute, 75189, Uppsala, Sweden

    Mikhayil Hakhverdyan

  3. Koltzov Institute of Developmental Biology, Russian Academy of Sciences, 119334, Moscow, Russia

    Irina Bakloushinskaya

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  1. Annette Baumstark
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  2. Horst Hameister
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  3. Mikhayil Hakhverdyan
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  4. Irina Bakloushinskaya
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  5. Walter Just
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Correspondence to Walter Just.

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Baumstark, A., Hameister, H., Hakhverdyan, M. et al. Characterization of Pisrt1/Foxl2 in Ellobius lutescens and exclusion as sex-determining genes. Mamm Genome 16, 281–289 (2005). https://doi.org/10.1007/s00335-004-2019-7

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