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A novel Krüppel-associated box identified in a panel of mammalian zinc finger proteins

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Abstract

Krüppel-related zinc finger proteins probably constitute the largest individual family of transcription factors in mammals. These proteins often carry a potent repressor domain called the Krüppel Associated Box (KRAB), which is known to effectively repress transcription through interaction with transcriptional intermediary factor 1β (TIF1β). Here we report the isolation and characterization of a novel human KRAB A zinc finger protein, HZF12. The gene encoding HZF12 is located on Chromosome (Chr) 19p13.11-p12, and a 4.4-kb transcript from this gene is expressed in a variety of adult and fetal tissues. Two additional, larger transcripts are expressed in testis only. Interestingly, the KRAB A domain of HZF12 is followed by a 21-amino acid domain, encoded by a separate exon. This domain, which we designate KRAB C, was also identified in more than 25 additional human, mouse, and rat KRAB zinc finger proteins. On the basis of results from a previous study, we conclude that this novel KRAB domain strengthens the interaction with TIF1β, thereby improving the ability of these KRAB zinc finger proteins to recruit TIF1β to specific sites.

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Acknowledgements

We thank Patrik Johansson (Uppsala University) for introducing us to JPred and NPS@. This work was supported by a grant from the European Commission (BIOMED2).

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Authors and Affiliations

  1. Department of Cell and Molecular Biology, Uppsala University, Uppsala, Sweden

    Camilla Looman & Lars Hellman

  2. Department of Veterinary Medical Chemistry, Swedish University of Agricultural Sciences, BMC, Box 575, SE-751 23 Uppsala, Sweden

    Magnus Åbrink

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  1. Camilla Looman
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  2. Lars Hellman
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  3. Magnus Åbrink
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Correspondence to Magnus Åbrink.

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Looman, C., Hellman, L. & Åbrink, M. A novel Krüppel-associated box identified in a panel of mammalian zinc finger proteins . Mamm Genome 15, 35–40 (2004). https://doi.org/10.1007/s00335-003-3022-0

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