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Serum leptin concentration is linked to Chromosomes 2 and 6 in the OLETF rat, an animal model of type 2 diabetes with mild obesity

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Abstract

Leptin is produced by adipose tissue and acts as a feedback signal to the hypothalamus controlling energy homeostasis, by reducing food consumption and increasing energy expenditure. Because serum leptin levels are highly correlated with body fat mass, they can be used as an index to predict obesity-related diseases. However, the identity of genetic factors that influence the obesity and the obesity-related metabolic disorders remains largely unknown. In this study, we performed a whole-genome scan search, using 382 F2 intercross progeny between the Otsuka Long-Evans Tokushima Fatty (OLETF) rat, an animal model for obese type 2 diabetes in human, and F344 rat, in order to identify loci responsible for the regulation of leptin and other obesity-related plasma substances. We have identified two quantitative trait loci (QTLs) contributing to serum leptin levels. These two loci, designated Olep1 [Chromosome (Chr) 2] and Olep2 (Chr 6), were homologous to those of human genome regions containing several potential candidate genes for obesity. These are fatty acid-binding protein 2 (FABP2), FABP4, and FABP5 for Olep1, and proopiomelanocortin (POMC) and glucose regulatory protein (GCKR) for Olep2.

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Acknowledgements

This study was supported in part by a grant from the Ministry of Education, Culture, Sports, Science & Technology of Japan. We thank Dr. Kazuya Kawano for kindly providing OLETF rats.

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Authors and Affiliations

  1. Institute for Animal Experimentation, University of Tokushima School of Medicine, Kuramoto 3, Tokushima 770-8503, Japan

    Tomoe Ogino, Daniel H. Moralejo, Hiroyuki Kose & Kozo Matsumoto

  2. Graduate School of Human Life Science, Osaka City University, 3-3-138 Sugimoto, Sumiyoshi, Osaka 558-8585, Japan

    Tomoe Ogino

  3. Department of Medicine, University of Washington, Seattle, Washington 98195-7710, USA

    Daniel H. Moralejo

  4. Laboratory of Animal Breeding and Genetics, Graduate School of Agriculture, Kyoto University, Sakyoku, Kyoto 606-8502, Japan

    Takahisa Yamada

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  1. Tomoe Ogino
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Correspondence to Kozo Matsumoto.

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Ogino, T., Moralejo, D.H., Kose, H. et al. Serum leptin concentration is linked to Chromosomes 2 and 6 in the OLETF rat, an animal model of type 2 diabetes with mild obesity . Mamm Genome 14, 239–244 (2003). https://doi.org/10.1007/s00335-003-2295-7

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  • DOI: https://doi.org/10.1007/s00335-003-2295-7

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