Noninvasive assessment of hepatic sinusoidal obstructive syndrome using acoustic radiation force impulse elastography imaging: A proof-of-concept study in rat models
- 247 Downloads
To determine the feasibility of acoustic radiation force impulse (ARFI) elastography in the evaluation of hepatic sinusoidal obstruction syndrome (SOS) in rat models.
Rat SOS models of various severities were created by monocrotaline gavage (n = 40) or by intraperitoneal injection of 5-fluorouracil, leucovorin and oxaliplatin (FOLFOX) (n = 16). Liver shear-wave velocity (SWV) was measured using ARFI elastography. Liver samples were analysed for the SOS score, steatosis, lobular inflammation and fibrosis.
The liver SWV was significantly elevated in the SOS models (1.29–2.24 m/s) compared with that of the matched control rats (1.01–1.09; p≤.09; veFor seven FOLFOX-treated rats which were longitudinally followed-up, the liver SWV significantly increased at 7 weeks (1.32±0.13 m/s) compared with the baseline (1.08±0.1 m/s, p=.015) and then significantly declined after a 2-week, treatment-free period (1.15±0.13 m/s; p=.048). Multivariate analysis revealed that the SOS score (p<.001) and lobular inflammation (p=.044) were independently correlated with the liver SWV.
Liver SWV is elevated in SOS in proportion to the degree of sinusoidal injury and lobular inflammation in rat SOS models. ARFI elastography has potential as an examination for diagnosis, severity assessment and follow-up of SOS.
• Liver SWV using ARFI elastography was significantly elevated in SOS rat.
• Sinusoidal injury and lobular inflammation grades had correlation with liver SWV.
• ARFI elastography has potential for diagnosis, severity assessment, and follow-up of SOS.
KeywordsLiver Sinusoidal obstruction syndrome Ultrasonography Acoustic radiation force impulse elastography Shear-wave velocity
Acoustic radiation force impulse
5-Fluorouracil, leucovorin and oxaliplatin combination
Haematoxylin and eosin
Sinusoidal obstruction syndrome
This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT and Future Planning (NRF-2017R1A2B4003114).
Compliance with ethical standards
The scientific guarantor of this publication is Moon Gyu Lee.
Conflict of interest
The authors of this manuscript declare no relationships with any companies whose products or services may be related to the subject matter of the article.
Statistics and biometry
One of the authors has significant statistical expertise.
Approval from the institutional animal care committee was obtained.
• performed at one institution
- 3.Mohty M, Malard F, Abecassis M et al (2015) Sinusoidal obstruction syndrome/veno-occlusive disease: current situation and perspectives-a position statement from the European Society for Blood and Marrow Transplantation (EBMT). Bone Marrow Transplant 50:781–789CrossRefPubMedPubMedCentralGoogle Scholar
- 30.Bano N (2013) Sinusoidal dilatations and splenomegaly as feature specifications of drug induced thromocytopenia in oxaliplatin treated Wistar rats. Asian Journal of Pharmaceutical and Clinical Research 6:262–264Google Scholar