Multispectral optoacoustic tomography of the human breast: characterisation of healthy tissue and malignant lesions using a hybrid ultrasound-optoacoustic approach

  • Anne Becker
  • Max Masthoff
  • Jing Claussen
  • Steven James Ford
  • Wolfgang Roll
  • Matthias Burg
  • Peter J. Barth
  • Walter Heindel
  • Michael Schäfers
  • Michel Eisenblätter
  • Moritz Wildgruber
Experimental

Abstract

Background and aim

Multispectral optoacoustic tomography (MSOT) represents a new in vivo imaging technique with high resolution (~250 μm) and tissue penetration (>1 cm) using the photoacoustic effect. While ultrasound contains anatomical information for lesion detection, MSOT provides functional information based on intrinsic tissue chromophores. We aimed to evaluate the feasibility of combined ultrasound/MSOT imaging of breast cancer in patients compared to healthy volunteers.

Methods

Imaging was performed using a handheld MSOT system for clinical use in healthy volunteers (n = 6) and representative patients with histologically confirmed invasive breast carcinoma (n = 5) and ductal carcinoma in situ (DCIS, n = 2). MSOT values for haemoglobin and oxygen saturation were assessed at 0.5, 1.0 and 1.5 cm depth and selected wavelengths between 700 and 850 nm.

Results

Reproducible signals were obtained in all wavelengths with consistent MSOT signals in superficial tissue in breasts of healthy individuals. In contrast, we found increased signals for haemoglobin in invasive carcinoma, suggesting a higher perfusion of the tumour and tumour environment. For DCIS, MSOT values showed only little variation compared to healthy tissue.

Conclusions

This preliminary MSOT breast imaging study provided stable, reproducible data on tissue composition and physiological properties, potentially enabling differentiation of solid malignant and healthy tissue.

Key Points

A handheld MSOT probe enables real-time molecular imaging of the breast.

MSOT of healthy controls provides a reproducible reference for pathology identification.

MSOT parameters allows for differentiation of invasive carcinoma and healthy tissue.

Keywords

Multispectral optoacoustic tomography Optoacoustic imaging Ultrasound In vivo imaging Breast cancer 

Abbreviations

DCIS

Ductal carcinoma in situ

MRI

Magnetic resonance imaging

MSOT

Multispectral optoacoustic tomography

OA

Optoacoustic

PET

Positron emission tomography

ROI

Region of interest

RUCT

Reflection ultrasound computed tomography

US

Ultrasound

Supplementary material

330_2017_5002_MOESM1_ESM.docx (97 kb)
ESM 1(DOCX 96 kb)

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Copyright information

© European Society of Radiology 2017

Authors and Affiliations

  • Anne Becker
    • 1
  • Max Masthoff
    • 1
  • Jing Claussen
    • 2
  • Steven James Ford
    • 2
  • Wolfgang Roll
    • 3
  • Matthias Burg
    • 1
  • Peter J. Barth
    • 4
  • Walter Heindel
    • 1
  • Michael Schäfers
    • 3
    • 5
  • Michel Eisenblätter
    • 1
    • 6
  • Moritz Wildgruber
    • 1
  1. 1.Department of Clinical RadiologyUniversity Hospital MuensterMuensterGermany
  2. 2.iThera MedicalMunichGermany
  3. 3.Department of Nuclear MedicineUniversity Hospital MuensterMuensterGermany
  4. 4.Gerhard-Domagk-Institute of PathologyUniversity Hospital MuensterMuensterGermany
  5. 5.European Institute for Molecular Imaging – EIMIUniversity of MuensterMuensterGermany
  6. 6.Divison of Imaging Sciences & Biomedical EngineeringKing’s College LondonLondonUK

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