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Usefulness of T1 mapping on Gd-EOB-DTPA-enhanced MR imaging in assessment of non-alcoholic fatty liver disease

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Abstract

Objectives

This study evaluates the value of Gd-EOB-DTPA-enhanced MRI for diagnosis and staging of non-alcoholic fatty liver disease (NAFLD) in an animal model by T1 relaxation time measurement.

Methods

Thirty-four rabbits were divided into the control group (n = 10) and NAFLD group, which was split into four groups (n = 6) with a high-fat diet for an interval of 3 weeks. A dual flip angle was performed before and at the hepatobiliary phase (HBP). T1 relaxation times of the liver parenchyma and the decrease rate (∆%) were calculated. Histological findings according to semi-quantitative scoring of steatosis, activity and fibrosis were the standard of reference.

Results

HBP and ∆% T1 relaxation time measurement showed significant differences between normal and NAFLD groups, between non-alcoholic steatohepatitis (NASH) and NAFLD without NASH (p = 0.000–0.049), between fibrosis groups (p = 0.000–0.019), but no difference between F1 and F2 (p = 0.834). The areas under the receiver operating characteristic curves (AUCs) of T1 relaxation time for HBP and ∆% were 0.86–0.93 for the selection of NASH and activity score ≥2, and 0.86–0.95 for the selection of F ≥ 1, 2, 3. No significant difference was found for diagnostic performance between HBP and ∆% T1 relaxation time.

Conclusions

HBP T1 relaxation time measurement of Gd-EOB-DTPA-enhanced MRI was useful to evaluate NAFLD according to the SAF score. HBP T1 relaxation time measurement was as accurate as ∆% T1 relaxation time.

Key Points

• Gd-EOB-DTPA-enhanced MRI could give useful information on NAFLD.

•HBP T 1 relaxation time measurement was useful for the evaluation of NAFLD.

• HBP T 1 relaxation time measurement was as accurate as ∆%.

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Abbreviations

AUC:

area under the ROC curve

∆%:

reduction rate

HE:

haematoxylin–eosin

HBP:

hepatobiliary phase

Gd-EOB-DTPA:

gadoxetic acid

LSD:

least significant difference

MRI:

magnetic resonance imaging

MRP:

multidrug resistance protein

NAFLD:

non-alcoholic fatty liver disease

NASH:

non-alcoholic steatohepatitis

OATP:

organic anion transporting polypeptide

ROI:

region of interest

ROC:

receiver operating characteristic

SAF:

steatosis (S), activity (A) and fibrosis (F)

SD:

standard deviation

VIBE:

volumetric interpolated breath-hold examination

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Acknowledgements

The scientific guarantor of this publication is Mengsu Zeng. The authors of this manuscript declare no relationships with any companies, whose products or services may be related to the subject matter of the article. This study received funding from the National Science Foundation for Young Scientists of China (Grant No. 81001025). One of the authors has significant statistical expertise. Institutional review board approval was obtained. Approval from the institutional animal care committee was obtained. No study subjects or cohorts have been previously reported.

Methodology: experimental study performed at one institution.

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Authors and Affiliations

  1. Department of Radiology, Zhongshan/Hospital of Fudan University, No 180/Fenglin Road, Xuhui District, Shanghai, China, 200032

    Ying Ding, Sheng-Xiang Rao, Tao Meng, Caizhong Chen, Renchen Li & Meng-Su Zeng

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  1. Ying Ding
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  2. Sheng-Xiang Rao
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Correspondence to Sheng-Xiang Rao.

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Ding, Y., Rao, SX., Meng, T. et al. Usefulness of T1 mapping on Gd-EOB-DTPA-enhanced MR imaging in assessment of non-alcoholic fatty liver disease. Eur Radiol 24, 959–966 (2014). https://doi.org/10.1007/s00330-014-3096-y

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  • DOI: https://doi.org/10.1007/s00330-014-3096-y

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