In vivo assessment of experimental neonatal excitotoxic brain lesion with USPIO-enhanced MR imaging
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To assess the feasibility of magnetic resonance imaging (MRI) enhanced with ultrasmall superparamagnetic particles of iron oxide (USPIO) for assessing excitotoxic brain lesions in an experimental model of neonatal periventricular white matter (PWM) lesions.
Materials and methods:
Brain lesions were induced by intracerebral injection of ibotenate in 14 newborn rats. Pre- and post-USPIO T2-weighted MRI was performed in seven of them (group A) and in five control newborns (group C). In seven newborns with induced cerebral lesions, USPIO-enhanced MRI was not performed (group B). We compared the signal intensity of the lesion to the contralateral unaffected brain (lesion-to-brain contrast, LBC) and the lesion signal-to-noise ratio (SNR) before and after USPIO injection. MR imaging was correlated with histology.
USPIO injection significantly (P < 0.05) decreased LBC and SNR of brain lesion but induced no changes in normal controls. The densities of macrophages and iron-laden cells were higher on the lesion side than on the contralateral side (P < 0.05). Neither lesion size nor the surrounding macrophage infiltrate was significantly different between groups A and B.
Post-USPIO T2-weighted MRI demonstrated negative enhancement of neonatal excitotoxic brain lesion. USPIO injection does not appear to exacerbate brain lesions.
KeywordsUSPIO Neuroinflammation Macrophage Periventricular white matter injury Cellular MRI
We are grateful to Mrs. Leslie Schendimann for technical support.
- 12.Dousset V, Ballarino L, Delalande C, Coussemacq M, Canioni P, Petry KG, Caille JM (1999) Comparison of ultrasmall particles of iron oxide (USPIO)-enhanced T2-weighted, conventional T2-weighted, and gadolinium-enhanced T1-weighted MR images in rats with experimental autoimmune encephalomyelitis. AJNR Am J Neuroradiol 20:223–227PubMedGoogle Scholar
- 16.Baeten K, Hendriks JJ, Hellings N, Theunissen E, Vanderlocht J, Ryck LD, Gelan J, Stinissen P, Adriaensens P (2008) Visualisation of the kinetics of macrophage infiltration during experimental autoimmune encephalomyelitis by magnetic resonance imaging. J Neuroimmunol 195:1–6CrossRefPubMedGoogle Scholar
- 26.Paxinos G, Watson C (2005) The rat brain in sterotaxic coordinates, 5th ed. Academic Press, New YorkGoogle Scholar
- 28.Chalard F, Daire JL, Plaisant P, Gressens P, Sebag G (2005) Application of in vivo magnetic resonance to newborn mouse model of excitotoxic periventricular leukomalacia. Pediatr Radiol 35(Suppl 2):101Google Scholar
- 29.Berger C, Hiestand P, Kindler-Baumann D, Rudin M, Rausch M (2006) Analysis of lesion development during acute inflammation and remission in a rat model of experimental autoimmune encephalomyelitis by visualization of macrophage infiltration, demyelination and blood-brain barrier damage. NMR Biomed 19:101–107CrossRefPubMedGoogle Scholar
- 38.Sigal R, Vogl T, Casselman J, Moulin G, Veillon F, Hermans R, Dubrulle F, Viala J, Bosq J, Mack M, Depondt M, Mattelaer C, Petit P, Champsaur P, Riehm S, Dadashitazehozi Y, de Jaegere T, Marchal G, Chevalier D, Lemaitre L, Kubiak C, Helmberger R, Halimi P (2002) Lymph node metastases from head and neck squamous cell carcinoma: MR imaging with ultrasmall superparamagnetic iron oxide particles (Sinerem MR)—results of a phase-III multicenter clinical trial. Eur Radiol 12:1104–1113CrossRefPubMedGoogle Scholar