Ultrastructure and proteins of the egg chorion of the antarctic fish Chionodraco hamatus (Teleostei, Notothenioidei)

Abstract.

The chorion morphology and protein content of unfertilised eggs of the antarctic icefish Chionodraco hamatus were characterised. Scanning and transmission electron microscopy showed that the chorion of this antarctic species is organised differently from that of non-polar species, with several concentric layers varying in thickness. Purified chorions were dissolved in 8-M urea buffer, and polypeptides were revealed by one- and two-dimensional gel electrophoresis. Glycoproteins were detected using affinity blotting with concanavalin-A. The principal glycoproteins had a molecular weight of 49 and 93 kDa. Purification of the main polypeptides between 40 and 92 kDa was achieved by preparative electrophoresis followed by electroelution of excised bands. The main biochemical composition of C. hamatus chorion was similar to that of other teleost species investigated.