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Polar Biology

, Volume 41, Issue 12, pp 2511–2521 | Cite as

Production of cold-adapted enzymes by filamentous fungi from King George Island, Antarctica

  • Alysson Wagner Fernandes Duarte
  • Mariana Blanco Barato
  • Fernando Suzigan Nobre
  • Danilo Augusto Polezel
  • Tássio Brito de Oliveira
  • Juliana Aparecida dos Santos
  • André Rodrigues
  • Lara Durães SetteEmail author
Original Paper
  • 227 Downloads

Abstract

Antarctic environments are characterized by polar climate, making it difficult for the development of any form of life. The biogeochemical cycles and food web in such restrictive environments may be exclusively formed by microorganisms. Polar mycological studies are recent and there is much to know about the diversity and genetic resources of these microorganisms. In this sense, the molecular taxonomic approach was applied to identify 129 fungal isolates from marine and terrestrial samples collected from the King George Island (South Shetland Islands, Maritime Antarctic). Additionally, the production of cold-adapted enzymes by these microorganisms was evaluated. Among the 129 isolates, 69.0% were identified by ITS-sequencing and affiliated into 12 genera. Cadophora, Geomyces, Penicillium, Cosmospora, and Cladosporium were the most abundant genera. Representatives of Cosmospora were isolated only from terrestrial samples, while representatives of the others genera were recovered from marine and terrestrial samples. A total of 29, 19, and 74 isolates were able to produce ligninolytic enzymes, xylanase, and l-asparaginase, respectively. Representatives of Cadophora showed great ability to produce lignin peroxidase (LiP) and laccase at 15.0 °C in liquid medium, while representatives of Penicillium and non-identified fungi were the best producers of xylanase and l-asparaginase at 20.0 °C. The high number of fungi able to produce enzymes at moderate temperature reveals their potential for industrial production and biotechnological applications. The present study broadens the knowledge of fungal diversity associated with the southern polar region. Additionally, data from molecular taxonomy suggest that two filamentous fungi may be considered as potential new species.

Keywords

Extremophiles Microbial biotechnology Ligninolytic enzymes l-Asparaginase Xylanase 

Notes

Acknowledgements

This study was financed by Fundação de Amparo à Pesquisa do Estado de São Paulo (Grants 2013/19486-0, 2016/07957-7). MBB and JAS thank the Coordenação de Aperfeiçoamento Pessoal de Nível Superior (CAPES) for their scholarships. LDS thanks the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for the Productivity Fellowship (303145/2016-1) and the Brazilian Antarctic Program (PROANTAR). The authors thank Professor Eduardo C. M. Hajdu and Dr. Itamar S. de Melo for the samplings of marine invertebrates and ornithogenic soil, respectively.

Compliance of ethical standards

Conflict of interest

The authors declare no conflict of interest.

Supplementary material

300_2018_2387_MOESM1_ESM.pdf (2.4 mb)
Supplementary material 1 (PDF 2418 kb) Electronic Supplementary Material: Online Resource 1. Table S1 Data related to the molecular identification of filamentous fungi from King George Island: numbers of pb analyzed and GenBank accession numbers; Fig. S1 Phylogenetic analysis of the sequences of fungi from King George Island, compared with type and closest sequences of the species following BLAST analysis. (A) Acremonium; (B) Cadophora; (C) Cercospora; (D) Cladosporium; (E) Cosmospora; (F) Geomyces and Pseudogymnoascus; (G) Oidiodendron; (H) Pseudeurotium; (I) Thelebolus. The trees were constructed based on the ITS region using MEGA v. 6.0 by the neighbor-joining algorithm, Kimura 2-parameters as the nucleotide substitution model and 1000 bootstrap pseudo-replicates (values lower than 50% are not shown). Sites containing gaps were excluded from the analysis. The scale bar indicates substitutions per site. All sequences were retrieved from GenBank (accessions are shown in parentheses). CBS (Centraalbureau voor Shimmelcutures), ATCC (American Type Culture Collection), IMI (International Mycological Institute), UAMH (University of Alberta Microfungus Collection and Herbarium) and MUCL (Mycothèque de l´Université Catholique de Louvain) voucher accessions are also shown. T: type strain; NT: neotype strain.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Alysson Wagner Fernandes Duarte
    • 1
  • Mariana Blanco Barato
    • 2
  • Fernando Suzigan Nobre
    • 2
  • Danilo Augusto Polezel
    • 3
  • Tássio Brito de Oliveira
    • 3
  • Juliana Aparecida dos Santos
    • 3
  • André Rodrigues
    • 3
  • Lara Durães Sette
    • 2
    • 3
    Email author
  1. 1.Federal University of AlagoasArapiracaBrazil
  2. 2.Division of Microbial ResourcesChemical, Biological and Agricultural Pluridisciplinary Research Center (CPQBA)/Campinas State University (UNICAMP)PaulíniaBrazil
  3. 3.Department of Biochemistry and Microbiology, Institute of BiosciencesSão Paulo State University (UNESP)Rio ClaroBrazil

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