Not only mosses: lemming winter diets as described by DNA metabarcoding
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The temporal dynamics of most tundra food webs are shaped by the cyclic population dynamics of lemmings. While processes during winter may be behind the recent disruptions of lemming cycles, lemming winter ecology is poorly known. We present here the first DNA metabarcoding data on the winter diet of Norwegian lemmings (Lemmus lemmus), based on feces collected after a winter of population increase. Prostrate willows, mosses, and graminoids dominated the species winter diet, indicating that the conventional idea of lemmings as moss-specialists should be revised. The behavior of lemming-plant models in theoretical studies is conditional on the assumptions of mosses being their main winter food item. As shrubs have been excluded from the framework of these models, incorporating them in future modeling studies should nuance our understanding on how plants affect lemmings. We also sampled diet of a few individuals found dead on top of the snow. These individuals had relatively empty stomachs and had, prior to death, relied heavily on mosses. This apparent lack of abundant good quality indicates spatial heterogeneity in local food availability during the population increase phase.
KeywordsArctic Bryophyte Lemmus lemmus Prostrate Salix Snowbed Winter
We thank Siw Killengreen, Geir Vie, Sandra Hamel, Kristoffer H. Juell, Francisco Javier Ancin Murguzur, and Ingrid Jensvoll for collecting samples in the field; Christian Miquel, Delphine Rioux and Alice Valentini help with the laboratory; Eric Coissac for help with the bioinformatics analyses; and Eivind Flittie Kleiven for help with checking snow melt dates. The work of Eeva M. Soininen was supported by Oskar Huttunen foundation and the Fram Centre through the Climate-Ecological Observatory for Arctic Tundra (COAT). Sample collection was funded by the Research Council of Norway (project EcoFinn).
Funding was provided by Oskar Huttunen Foundation (Grant for doctoral studies) and Norwegian Research Council (Grant No. 184140).
Compliance with ethical standards
Conflict of interest
We would like to mention that Ludovic Gielly is one of the co-inventors of a patent concerning g-h primers and the subsequent use of the P6 loop of the chloroplast trnL (UAA) intron for plant identification using degraded template DNA. These patents only restrict commercial applications and have no impact on the use of this locus by academic researchers.
- Ims RA, Jepsen JU, Stien A, Yoccoz NG (2013) Science plan for COAT: climate ecological observatory for Arctic Tundra. Fram Centre Report Series, vol. 1. Fram Centre, TromsøGoogle Scholar
- Kalela O, Koponen T, Lind EA, Skarén U, Tast J (1961) Seasonal change of habitat in the Norwegian lemming Lemmus lemmus (L.). Ann Acad Sci Fenn A4 55:1–72Google Scholar
- Koshkina TV (1961) New data on the nutrition of the Norwegian lemming (Lemmus lemmus) (in Russian). Byulletin Moskouskogo Obschchestua Ispyt Prir Otdel Biol 66:15–31Google Scholar
- Newmaster SG, Thompson ID, Steeves RAD, Rodgers AR, Fazekas AJ, Maloles JR, McMullin RT, Fryxell JM (2013) Examination of two new technologies to assess the diet of woodland caribou: video recorders attached to collars and DNA barcoding. Can J For Res 43(10):897–900. doi: 10.1139/cjfr-2013-0108 CrossRefGoogle Scholar
- Oksanen T, Oksanen L, Dahlgren J, Olofsson J (2008) Arctic lemmings, Lemmus spp. and Dicrostonyx spp.: integrating ecological and evolutionary perspectives. Evol Ecol Res 10:415–434Google Scholar
- Soininen EM (2012) Interactions between small rodents and their food plants in tundra habitats (PhD thesis). University of Tromsø, Tromsø, NorwayGoogle Scholar
- Soininen EM, Valentini A, Coissac E, Miquel C, Gielly L, Brochmann C, Brysting AK, Sonstebo JH, Ims RA, Yoccoz NG, Taberlet P (2009) Analysing diet of small herbivores: the efficiency of DNA barcoding coupled with high-throughput pyrosequencing for deciphering the composition of complex plant mixtures. Front Zool 6:16. doi: 10.1186/1742-9994-6-16 CrossRefPubMedPubMedCentralGoogle Scholar
- Soininen EM, Zinger L, Gielly L, Bellemain E, Bråthen KA, Brochmann C, Epp LS, Gussarova G, Hassel K, Henden J-A, Killengreen ST, Rämä T, Stenøien HK, Yoccoz NG, Ims RA (2013) Shedding new light on the diet of Norwegian lemmings: DNA metabarcoding of stomach content. Polar Biol 36:1069–1076CrossRefGoogle Scholar
- Soininen EM, Ehrich D, Lecomte N, Yoccoz NG, Tarroux A, Berteaux D, Gauthier G, Gielly L, Brochmann C, Gussarova G, Ims RA (2014) Sources of variation in small rodent trophic niche: new insights from DNA metabarcoding and stable isotope analysis. Isot Environ Healt S 50 (3):361–381CrossRefGoogle Scholar
- Soininen EM, Gauthier G, Bilodeau F, Berteaux D, Gielly L, Taberlet P, Gussarova G, Bellemain E, Hassel K, Stenøien HK, Epp L, Schrøder-Nielsen A, Brochmann C, Yoccoz NG (2015a) Highly overlapping winter diet in two sympatric lemming species revealed by DNA metabarcoding. PLoS ONE 10(1):e0115335. doi: 10.1371/journal.pone.0115335 CrossRefPubMedPubMedCentralGoogle Scholar
- Soininen EM, Gauthier G, Bilodeau F, Berteaux D, Gielly L, Taberlet P, Gussarova G, Bellemain E, Hassel K, Stenøien HK, Epp L, Schrøder-Nilsen A, Brochmann C, Yoccoz NG (2015b) Data from: highly overlapping winter diet in two sympatric lemming species revealed by DNA metabarcoding. Dryad Data Repos. doi: 10.5061/dryad.4rr39 Google Scholar