Marine archaeal community structure from Potter Cove, Antarctica: high temporal and spatial dominance of the phylum Thaumarchaeota
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Archaeal communities represent a significant fraction of the Antarctic marine microbial plankton and surely play a relevant role in the proper functioning of the ecosystem. We studied the archaeal community structure in surface water samples from Potter Cove, Antarctica. Temporal and spatial variability was investigated along a whole year cycle using DGGE and 16S rRNA gene sequencing from clone libraries. Additionally, photosynthetic pigments, suspended particulate matter (SPM), salinity and temperature were measured. The multivariate analysis performed using diversity, dominance and richness indexes, and environmental data evidenced a seasonal pattern in the archaeal community and revealed that spring–summer samples clustered separately from autumn to winter ones. High salinity and high values of diversity and richness were related to autumn–winter samples, whereas the spring–summer samples were associated mainly with higher values of temperature, SPM, Chl-a, carotenoids and archaeal dominance. The phylogenetic analysis of five independent clone libraries (467 sequences) showed that 448 sequences fell into a clade containing Nitrosopumilus maritimus and other sequences of ammonia-oxidizing archaea which belong to the Thaumarchaeota phylum. A high fraction of these sequences (62 %) constituted a single cluster containing only highly similar Potter Cove representatives, which probably belong to the same species. Fifteen sequences were affiliated to a group closely related to the order Thermoplasmatales (Euryarchaeota). This work represents a first step towards obtaining a deep understanding of the structure of archaeal communities from Antarctic coastal marine environments and contributes to cover the current gap in knowledge of the dynamics of the archaeoplankton in the Antarctic seas.
KeywordsAntarctic Peninsula 16S rDNA DGGE Marine archaea
This research was carried out under an agreement between the Instituto Antártico Argentino and the Facultad de Farmacia y Bioquímica of the Universidad de Buenos Aires. This work was supported by grants PICTO 2010-0124 from the Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT) and UBA 20020100100378 from Universidad de Buenos Aires. Also we had the financial support from the European Commission through the Marie Curie Action IRSES, project no 318718, IMCONet (Interdisciplinary Modelling of climate change in coastal Western Antarctica—Network for staff Exchange and Training). We thank Gustavo Latorre, Gastón Aguirre and Oscar Gonzalez for their technical assistance and Cecilia Ferreiro for the correction of the English manuscript.
- Amano-Sato C, Akiyama S, Uchida M, Shimada K, Utsumi M (2013) Archaeal distribution and abundance in water masses of the Arctic Ocean, Pacific sector. Aquat Microb Ecol 69:101–112Google Scholar
- Collins RE, Rocap G, Deming JW (2010) Persistence of bacterial and archaeal communities in sea ice through an Arctic winter. Environ Microbiol 12:1828–1841Google Scholar
- Di Rienzo JA, Casanoves F, Balzarini MG, Gonzalez L, Tablada M, Robledo CW (2001) InfoStat versión 2011. Grupo InfoStat, FCA, Universidad Nacional de Córdoba, Argentina. http://www.infostat.com.ar
- Fuenets VL, Schnack-Schiel SB, Schloss IR, Esnal GG (2008) Mesozooplankton of Potter Cove: community composition and seasonal distribution in 2002–2003. Rep Pol Mar Res 571:75–84Google Scholar
- Hallam SJ, Mincer TJ, Schleper C, Preston CM, Roberts K, Richardson PM, DeLong EF (2006) Pathways of carbon assim-ilation and ammonia oxidation suggested by environmental genomic analyses of marine Crenarchaeota. PLoS Biol 4:520–536, E95. doi: 10.1371/journal.pbio.0040095
- Holland SM (2003) Analytic rarefaction. See http://www.uga.edu/strata/software/
- JGI (Joint Genome Institute) (2013) https://img.jgi.doe.gov/cgi-bin/mer/main.cgi?section=TaxonDetail&page=taxonDetail&taxon_oid=3300000136
- Muyzer G, de Waal EC, Uitterlinden AG (1993) Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rDNA. Appl Environ Microb 59:695–700Google Scholar
- Muyzer G, Brinkhoff T, Nübel U, Santegoeds C, Schäfer H, Wawer C (2004) Denaturing gradient gel electrophoresis (DGGE) in microbial ecology. In: Kowalchuk GA, de Bruin FJ, Head IM, Akkermans DL, Van Elsas JD (eds) Molecular microbial ecology manual. Kluwer, Dordrecht, pp 743–770Google Scholar
- Strickland JDH, Parsons TR (1968) A practical handbook of seawater analysis. Pigment analysis, Bull Fish Res Bd, Otawa, Canada, 167, 311 ppGoogle Scholar
- Strickland JDH, Parsons DR (1972) A practical handbook of seawater analysis. J Fish Res Board Can Bull 167:1–310Google Scholar