Polar Biology

, Volume 37, Issue 8, pp 1133–1144 | Cite as

Transcriptome information of the Arctic green sea urchin and its use in environmental monitoring

  • Jae-Sung Rhee
  • Bo-Mi Kim
  • Beom-Soon Choi
  • Ik-Young Choi
  • Hyun Park
  • In-Young Ahn
  • Jae-Seong Lee
Original Paper


The Arctic sea urchin Strongylocentrotus droebachiensis is considered to be a potential indicator species for assessing aquatic environmental conditions in the polar region. To develop a gene resource for the Arctic sea urchin, we sequenced S. droebachiensis cDNAs using a GS-20 sequencer and obtained expressed sequence tags information. In the assembly stage, 31,526 transcripts were obtained and showed a 32 % hit rate in the GenBank non-redundant database with 9,995 unigenes. Of the transcripts obtained in this species, we found several stress- and cellular defense-related genes (antioxidant-related genes and genes from the heat shock protein family) that are useful for the monitoring of pollutant-triggered stress responses at the molecular level. To validate the usefulness of these potential biomarker genes, we analyzed the transcript profiles of selected genes in response to polychlorinated biphenyls (PCB) mixtures (Aroclor 1254) for 48 h. PCB contamination is a present-day threat to the health of individual organisms and ecosystems in the polar region. We showed that 11 of 14 genes responded to PCB treatment at transcriptional levels, with the most dramatic upregulation observed in the hsp70.5 gene. In this paper, we summarize the expressed cDNA information and discuss its potential use in ecotoxicological genomic studies on PCB exposure.


Arctic sea urchin Strongylocentrotus droebachiensis Expressed sequence tag Polychlorinated biphenyls Aroclor 1254 



We thank Dr. Hans-U. Dahms for his comments on the manuscript and also thank three anonymous reviewers for their valuable comments. This work was supported by a grant from KOPRI (PE08040) and also supported by a grant of K-POD (2014) funded to Jae-Seong Lee.

Supplementary material

300_2014_1507_MOESM1_ESM.xlsx (7.2 mb)
Supplementary material 1 (XLSX 7365 kb)


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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Jae-Sung Rhee
    • 1
  • Bo-Mi Kim
    • 2
  • Beom-Soon Choi
    • 3
  • Ik-Young Choi
    • 3
  • Hyun Park
    • 4
  • In-Young Ahn
    • 5
  • Jae-Seong Lee
    • 2
  1. 1.Department of Marine Science, College of Natural SciencesIncheon National UniversityIncheonSouth Korea
  2. 2.Department of Biological Sciences, College of ScienceSungkyunkwan UniversitySuwonSouth Korea
  3. 3.National Instrumentation Center for Environmental Management, College of Agriculture and Life SciencesSeoul National UniversitySeoulSouth Korea
  4. 4.Division of Polar Life SciencesKorea Polar Research InstituteIncheonSouth Korea
  5. 5.Division of Polar Ocean EnvironmentKorea Polar Research InstituteIncheonSouth Korea

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