Abstract
Transgenic yellow lupin (Lupinus luteus L.) plants have been generated by meristem co-cultivation with Agrobacterium tumefaciens. The binary plasmid pPZBNIa contains the bar gene under the control of a CaMV 35 S promoter. The transformation method involves inoculation of embryonic axis explants with A. tumefaciens, flooding the meristem with glufosinate, and initial culture on non-selective medium. Shoots were transferred to culture medium containing 20 mg/l glufosinate. Following subculture, shoots were grafted onto non-transgenic narrow-leafed lupin (L. angustifolius L.) seedling rootstocks, or rooted in vitro. The overall transformation efficiency, as determined at the T1 generation, was 0.05%–0.75%. The transgenic nature of plants grown to the T6 generation was confirmed by phosphinothricin acetyl transferase, PCR and Southern analyses.
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Received: 20 March 1999 / Revision received: 17 July 1999 / Accepted: 17 August 1999
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Li, H., Wylie, S. & Jones, M. Transgenic yellow lupin (Lupinus luteus). Plant Cell Reports 19, 634–637 (2000). https://doi.org/10.1007/s002990050785
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DOI: https://doi.org/10.1007/s002990050785