Abstract
In an attempt to understand the molecular basis of plant responses to wounding, we have investigated the transcriptional regulation of the wound-inducible prxC2 gene, which encodes horseradish peroxidase. In the previous work, 5′-deletion analysis revealed that a cis-element involved in the expression of the prxC2 gene is located at a position between –296 and –283 bp from the translation start site and contains a G-box sequence. We have also reported that a trans-acting factor, TFHP-1, can bind to the G-box sequence in this cis-element. Although the antisense RNA of TFHP-1 suppressed the expression of a prxC2 promoter-GUS chimeric gene in tobacco protoplasts, the sense RNA of TFHP-1 could not enhance this expression. These results suggested that other elements function in the expression of the prxC2 gene, and therefore additional deletion analysis of the promoter was performed with a transient expression system in cultured tobacco cells. Consequently, the second cis-element was found at a position between –177 and –134 bp from the translation start site of prxC2. This second cis-element contains a sequence similar to a PAL-box, and a nuclear protein possibly binds to it through the PAL-box sequence.
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Received: 18 January 1999 / Revision received: 19 August 1999 / Accepted: 24 August 1999
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Kaothien, P., Shimokawatoko, Y., Kawaoka, A. et al. A cis-element containing PAL-box functions in the expression of the wound-inducible peroxidase gene of horseradish. Plant Cell Reports 19, 558–562 (2000). https://doi.org/10.1007/s002990050773
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DOI: https://doi.org/10.1007/s002990050773