Skip to main content
SpringerLink
Log in

Agrobacterium tumefaciens-mediated transformation of Robinia pseudoacacia

  • Original paper
  • Published:
Plant Cell Reports Aims and scope Submit manuscript

Robinia pseudoacacia

(black locust) plants were regenerated after co-cultivation of stem and leaf segments with Agrobacterium tumefaciens strain GV3101 (pMP90) that harbored a binary vector that included genes for β-glucuronidase (GUS) and hygromycin phosphotransferase. Successful transformation was confirmed by the ability of stem and leaf segments to produce calli in the presence of hygromycin, by histochemical and fluorometric assays of GUS activity in plant tissues, and by Southern blotting analysis. In this transformation system, about 2 months were required for regeneration of transgenic plants from stem and leaf segments. The frequency of transformation from stem segments was approximately 24%, and the morphology of regenerated plants resembled that of the original parental strain.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Author information

Authors and Affiliations

  1. Molecular and Cell Biology Section, Bio-Resources Technology Division, Forestry and Forest Products Research Institute, Ibaraki 305-8687, Japan Fax: +81-298-73-0507 e-mail: kenjis@ffpri.affrc.go.jp, , , , , , JP

    T. Igasaki, T. Mohri & K. Shinohara

  2. Department of Biotechnology, National Institute of Agrobiological Resources, Ibaraki 305-8602, Japan, , , , , , JP

    H. Ichikawa

Authors
  1. T. Igasaki
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. T. Mohri
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. H. Ichikawa
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. K. Shinohara
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Received: 2 September 1999 / Revision received: 30 November 1999 / Accepted: 4 December 1999

Rights and permissions

Reprints and permissions

About this article

Cite this article

Igasaki, T., Mohri, T., Ichikawa, H. et al. Agrobacterium tumefaciens-mediated transformation of Robinia pseudoacacia . Plant Cell Reports 19, 448–453 (2000). https://doi.org/10.1007/s002990050754

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/s002990050754

Search

Navigation