Abstract
A cryopreservation procedure using encapsulation/dehydration was established for shoot-tips obtained from in vitro-grown shoots of hop. After dissection, shoot-tips were encapsulated in medium with alginate and 0.5 M sucrose. Optimal conditions consisted of preculture for 2 days in solid medium with 0.75 M sucrose, or in increasing sucrose concentrations, desiccation for 4 h with silicagel in a flow cabinet (16% water content) followed by rapid freezing and slow thawing. Shoot recovery after freezing 60 min in liquid nitrogen was around 80%. No phenotypical changes were observed in the recovered plants from cryopreserved shoot-tips growing in the field.
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Received: 20 April 1997 / Revised: 20 February 1998 / Accepted: 1 Dezember 1998
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Martínez, D., Tamés, R. & Angeles Revilla, M. Cryopreservation of in vitro-grown shoot-tips of hop (Humulus lupulus L.) using encapsulation/dehydration. Plant Cell Reports 19, 59–63 (1999). https://doi.org/10.1007/s002990050710
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DOI: https://doi.org/10.1007/s002990050710