Abstract
A cryopreservation procedure using encapsulation/dehydration was established for shoot-tips obtained from in vitro-grown shoots of hop. After dissection, shoot-tips were encapsulated in medium with alginate and 0.5 M sucrose. Optimal conditions consisted of preculture for 2 days in solid medium with 0.75 M sucrose, or in increasing sucrose concentrations, desiccation for 4 h with silicagel in a flow cabinet (16% water content) followed by rapid freezing and slow thawing. Shoot recovery after freezing 60 min in liquid nitrogen was around 80%. No phenotypical changes were observed in the recovered plants from cryopreserved shoot-tips growing in the field.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Explore related subjects
Discover the latest articles and news from researchers in related subjects, suggested using machine learning.Author information
Authors and Affiliations
Additional information
Received: 20 April 1997 / Revised: 20 February 1998 / Accepted: 1 Dezember 1998
Rights and permissions
About this article
Cite this article
Martínez, D., Tamés, R. & Angeles Revilla, M. Cryopreservation of in vitro-grown shoot-tips of hop (Humulus lupulus L.) using encapsulation/dehydration. Plant Cell Reports 19, 59–63 (1999). https://doi.org/10.1007/s002990050710
Issue Date:
DOI: https://doi.org/10.1007/s002990050710