Abstract
A transformation/regeneration system was developed for the common garden Lobelia (Lobelia erinus). Using an Agrobacterium-based protocol, over 40 transformants have been generated with four different binary vectors. The explant source was hypocotyl-root sections from axenically grown seedlings. Stable transformation was demonstrated by Southern hybridization analysis, β-glucuronidase staining, and transmission of the T-DNA to progeny. This extends the ever-widening range of transformable plant species to the Campanulaceae and will allow molecular studies of development and physiology in this easily cultured and popular garden plant.
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Received: 16 September 1997 / Revision received: 3 June 1998 / Accepted: 14 July 1998
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Payne, T., Lloyd, A. Transformation and regeneration of Lobelia erinus using Agrobacterium tumefaciens. Plant Cell Reports 18, 308–311 (1998). https://doi.org/10.1007/s002990050577
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DOI: https://doi.org/10.1007/s002990050577