Skip to main content

Development of an efficient Agrobacterium-mediated transformation system for Brassica carinata

Abstract

Shoot organogenesis and plant regeneration were readily achieved from cotyledonary petioles and hypocotyls of Brassica carinata. These explants were used for Agrobacterium-mediated transformation. A construct containing the selectable marker genes, neomycin phosphotransferase II, phosphinothricin acetyl transferase and the reporter gene β-glucuronidase, under the control of a tandem 35S promoter, was used for transformation. Although transformation was achieved with both cotyledonary petioles and hypocotyls, cotyledonary petioles responded best, with 30–50% of the explants producing GUS-positive shoots after selection on 25 mg/l kanamycin. Direct selection on L-phosphinothricin also produced resistant shoots but at a lower frequency (1–2%).

This is a preview of subscription content, access via your institution.

Author information

Authors and Affiliations

Authors

Additional information

Received: 9 April 1997 / Revision received: 3 July 1997 / Accepted: 30 July 1997

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Babic, V., Datla, R., Scoles, G. et al. Development of an efficient Agrobacterium-mediated transformation system for Brassica carinata. Plant Cell Reports 17, 183–188 (1998). https://doi.org/10.1007/s002990050375

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/s002990050375

  • Key words Brassica
  • Mustard
  • Transgenic
  • Transformation
  • Regeneration