Skip to main content
Log in

Vacuum infiltration of Petunia hybrida pollen with Agrobacterium tumefaciens to achieve plant transformation

  • Published:
Plant Cell Reports Aims and scope Submit manuscript


 Genetic transformation of Petunia hybrida with a reporter gene and selectable marker gene (35S-bar) was achieved in similar frequencies by pollinating flowers with pollen vacuum-infiltrated with Agrobacterium tumefaciens or applying a drop of Agrobacterium suspension to the stigma immediately prior to pollination. Nine percent of the T1, and 5% of the T2 progeny germinated in nutrient medium with 3 mgl/l BastaR. Polymerase chain reaction assays indicated that of the BastaR-resistant plants, 66% of the T1 plants, and 61% of the T2 plants harboured the GUS gene. Histochemical assays showed that 10% of the putatively transformed T1 plants and 5% of their progeny expressed GUS in leaf tissue, pistils and young anthers. Southern hybridization confirmed genomic integration of the bar gene in one to three places in selected T1 and T2 progeny.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

Author information

Authors and Affiliations


Additional information

Received: 12 March 1999 / Revision received: 1 October 1999 / Accepted: 20 October 1999

Rights and permissions

Reprints and permissions

About this article

Cite this article

Tjokrokusumo, D., Heinrich, T., Wylie, S. et al. Vacuum infiltration of Petunia hybrida pollen with Agrobacterium tumefaciens to achieve plant transformation. Plant Cell Reports 19, 792–797 (2000).

Download citation

  • Issue Date:

  • DOI: