Abstract
Embryo axes of four accessions of chickpea (Cicer arietinum L.) were treated with Agrobacterium tumefaciens strains C58C1/GV2260 carrying the plasmid p35SGUSINT and EHA101 harbouring the plasmid pIBGUS. In both vectors the GUS gene is interrupted by an intron. After inoculation shoot formation was promoted on MS medium containing 0.5 mg/l BAP under a selection pressure of 100 mg/l kanamycin or 10 mg/l phosphinothricin, depending on the construct used for transformation. Expression of the chimeric GUS gene was confirmed by histochemical localization of GUS activity in regenerated shoots. Resistant shoots were grafted onto 5-day-old dark-grown seedlings, and mature plants could be recovered. T-DNA integration was confirmed by Southern analysis by random selection of putative transformants. The analysis of 4 plantlets of the T1 progeny revealed that none of them was GUS-positive, whereas the presence of the nptII gene could be detected by polymerase chain reaction.
Similar content being viewed by others
Author information
Authors and Affiliations
Additional information
Received: 30 May 1997 / Revision received: 18 September 1997 / Accepted: 22 March 1999
Rights and permissions
About this article
Cite this article
Krishnamurthy, K., Suhasini, K., Sagare, A. et al. Agrobacterium mediated transformation of chickpea (Cicer arietinum L.) embryo axes. Plant Cell Reports 19, 235–240 (2000). https://doi.org/10.1007/s002990050005
Issue Date:
DOI: https://doi.org/10.1007/s002990050005