Sugar-regulated control of α-tubulin in maize cell suspension culture

Abstract.

The data reported here shows that α-tubulin, the subunit of microtubulin in the cytoskeleton, is regulated by sugars. A 48-h sugar-depletion treatment in BMS maize cell suspension culture medium led to a marked reduction in the levels of α-tubulin mRNA and protein. This response was reversible; the addition of metabolizable sugars led to a rapid increase in the levels of the α-tubulin transcript. Levels of α-tubulin protein also increased, albeit gradually. Surprisingly, there was little or no effect of phosphate depletion and phosphate addition on α-tubulin expression. Furthermore, the data show that sugars modulate a co-regulation of α-tubulin and cell-wall invertase. We propose that microtubules might be a major component of a sugar-signaling pathway that is regulated by, among other factors, cellular carbon metabolism.