Abstract
Two-dimensional fluorescence spectroscopy (2D-FS) has been used as a new method for determining the viability of tobacco cells (Nicotiana tabacum L.). Both horizontal beam geometry and a vertical set-up achieved with bifurcated fibres were tested. The latter arrangement enabled us to avoid the negative effect of cell sedimentation. Incubation of a tobacco BY-2 cell suspension with dimethylsulfoxide (DMSO) (0–10% v/v) resulted in cell samples differing in their viability – from fully viable (0–2% DMSO) to totally non-viable (8–10%DMSO). The validity of determining viability by means of measuring cell esterase activity by 2D-FS using fluorescein diacetate as a fluorogenic substrate was verified by comparison with microscopic evaluation of fluorescein fluorescence as well as with the routinely adopted trypan blue exclusion test.
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Received: 6 June 2000 / Revision received: 9 October 2000 / Accepted: 9 October 2000
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Vaňková, R., Kuncová, G., Opatrná, J. et al. Two-dimensional fluorescence spectroscopy – a new tool for the determination of plant cell viability. Plant Cell Reports 20, 41–47 (2001). https://doi.org/10.1007/s002990000294
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DOI: https://doi.org/10.1007/s002990000294