A new way of achieving plant regeneration of Solanum lycopersicoides Dun. from root primordia culture

Abstract

Cell aggregates with root primordia were formed in root primordia culture (RPC) of Solanum lycopersicoides grown in modified liquid MS medium containing 15 mg/l NAA. After transfer to liquid medium containing 1 mg/l 2,4-D, the aggregates dissociated into single root primordia (RP) which had an organizing root meristem at the apical pole. Oval structures called pseudoembryos were formed from single RP. After passage to liquid MS medium without phyto-hormones and organic compounds (with the exception of sucrose), an apical root meristem developed and the shoot apical meristem was initiated. The pseudoembryos developed into elongated pseudoseedlings which formed plants after transfer to a 1/2MSV medium. The development of pseudoembryos occurred without the callus phase. Moreover, the induction of the shoot meristem occurred without exogenous cytokinins.