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Sugarcane ScOPR1 gene enhances plant disease resistance through the modulation of hormonal signaling pathways

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Abstract

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Transgenic plants stably overexpressing ScOPR1 gene enhanced disease resistance by increasing the accumulation of JA, SA, and GST, as well as up-regulating the expression of genes related to signaling pathways.

Abstract

12-Oxo-phytodienoate reductase (OPR) is an oxidoreductase that depends on flavin mononucleotide (FMN) and catalyzes the conversion of 12-oxophytodienoate (12-OPDA) into jasmonic acid (JA). It plays a key role in plant growth and development, and resistance to adverse stresses. In our previous study, we have obtained an OPR gene (ScOPR1, GenBank Accession Number: MG755745) from sugarcane. This gene showed positive responses to methyl jasmonate (MeJA), salicylic acid (SA), abscisic acid (ABA), and Sporisorium scitamineum, suggesting its potential for pathogen resistance. Here, in our study, we observed that Nicotiana benthamiana leaves transiently overexpressing ScOPR1 exhibited weaker disease symptoms, darker 3,3-diaminobenzidine (DAB) staining, higher accumulation of reactive oxygen species (ROS), and higher expression of hypersensitive response (HR) and SA pathway-related genes after inoculation with Ralstonia solanacearum and Fusarium solanacearum var. coeruleum. Furthermore, the transgenic N. benthamiana plants stably overexpressing the ScOPR1 gene showed enhanced resistance to pathogen infection by increasing the accumulation of JA, SA, and glutathione S-transferase (GST), as well as up-regulating genes related to HR, JA, SA, and ROS signaling pathways. Transcriptome analysis revealed that the specific differentially expressed genes (DEGs) in ScOPR1-OE were significantly enriched in hormone transduction signaling and plant–pathogen interaction pathways. Finally, a functional mechanism model of the ScOPR1 gene in response to pathogen infection was depicted. This study provides insights into the molecular mechanism of ScOPR1 and presents compelling evidence supporting its positive involvement in enhancing plant disease resistance.

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Funding

This study was supported by the Special Projects for the Central-guided Local Science and Technology Development (2022L3086), National Key Research and Development Program of China (2022YFD2301100), China Agriculture Research System of MOF and MARA (CARS-17), Project of National Key Laboratory for Tropical Crop Breeding (1630052024003 and NKLTCB-YAAS-2024-S01), Director’s Research Fund of National Engineering Research Center for sugarcane, Fujian Agriculture and Forestry University (NERD202212), and Natural Science Foundation of Fujian Province, China (2021J01137 and 2020J01591).

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Wenhui Zou: data curation, investigation, methodology, software, validation, visualization, writing—original draft; Tingting Sun: data curation, funding acquisition, investigation, methodology, software, validation, visualization, writing—original draft; Yao Chen: data curation, investigation, software, validation, visualization; Dongjiao Wang: data curation, investigation, validation, visualization; Chuihuai You: data curation, investigation, software; Shoujian Zang: data curation, validation, visualization; Peixia Lin: data curation, investigation, software; Qibin Wu: conceptualization, project administration, supervision, writing—review and editing; Yachun Su: conceptualization, funding acquisition, supervision, writing—review and editing; Youxiong Que: conceptualization, funding acquisition, project administration, supervision, writing—review and editing.

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Correspondence to Qibin Wu, Yachun Su or Youxiong Que.

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Communicated by Leandro Peña.

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Zou, W., Sun, T., Chen, Y. et al. Sugarcane ScOPR1 gene enhances plant disease resistance through the modulation of hormonal signaling pathways. Plant Cell Rep 43, 158 (2024). https://doi.org/10.1007/s00299-024-03241-8

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  • DOI: https://doi.org/10.1007/s00299-024-03241-8

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