Skip to main content
Log in

Comparative proteomic and metabolomic analyses reveal stress responses of hemp to salinity

  • Original Article
  • Published:
Plant Cell Reports Aims and scope Submit manuscript

Abstract

Key message

Integrated omics analyses outline the cellular and metabolic events of hemp plants in response to salt stress and highlight several photosynthesis and energy metabolism related pathways as key regulatory points.

Abstract

Soil salinity affects many physiological processes of plants and leads to crop yield losses worldwide. For hemp, a crop that is valued for multiple aspects, such as its medical compounds, fibre, and seed, a comprehensive understanding of its salt stress responses is a prerequisite for resistance breeding and tailoring its agronomic performance to suit certain industrial applications. Here, we first observed the phenotype of salt-stressed hemp plants and found that under NaCl treatment, hemp plants displayed pronounced growth defects, as indicated by the significantly reduced average height, number of leaves, and chlorophyll content. Next, we conducted comparative proteomics and metabolomics to dissect the complex salt-stress response mechanisms. A total of 314 proteins and 649 metabolites were identified to be differentially behaving upon NaCl treatment. Functional classification and enrichment analysis unravelled that many differential proteins were proteases associated with photosynthesis. Through metabolic pathway enrichment, several energy-related pathways were found to be altered, such as the biosynthesis and degradation of branched-chain amino acids, and our network analysis showed that many ribosomal proteins were involved in these metabolic adaptations. Taken together, for hemp plants, influences on chloroplast function probably represent a major toxic effect of salinity, and modulating several energy-producing pathways possibly through translational regulation is presumably a key protective mechanism against the negative impacts. Our data and analyses provide insights into our understanding of hemp’s stress biology and may lay a foundation for future functional genomics studies.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

Availability of data and materials

The datasets supporting the conclusions of the present study are included within this article and the supplementary material.

References

Download references

Acknowledgements

‘Bama’ hemp seeds were kindly provided by the Guangxi Academy of Agricultural Sciences in China. We thank Prof. Feihu Liu (School of Agriculture, Yunnan University) for helpful advice on the experimental design. Z. Li also acknowledges the financial aid of grants from Research Start-Up Funding of Yunnan University (C176220100077), Yunnan Fundamental Research Projects (202201AT070130 and 202301AT070168), and the National Natural Science Foundation of China (32360448).

Funding

This study was funded by the Scientific Research Fund of the Yunnan Provincial Education Department (2023J0005) and the National Natural Science Foundation of China (32360538).

Author information

Authors and Affiliations

Authors

Contributions

Y.Y, G.D, and Z.Li. designed and supervised the project. Y.Y. and H.Y. performed the experiments. Z.Li, Y.C., and Z.Lu. analysed the data. Z.Li. and Y.Y. wrote, reviewed, and edited the manuscript. All authors read and approved the manuscript.

Corresponding author

Correspondence to Zheng Li.

Ethics declarations

Conflict of interest

The authors have no relevant financial or non-financial interests to disclose.

Additional information

Communicated by Leandro Peña.

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary Information

Rights and permissions

Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Yang, Y., Cheng, Y., Lu, Z. et al. Comparative proteomic and metabolomic analyses reveal stress responses of hemp to salinity. Plant Cell Rep 43, 154 (2024). https://doi.org/10.1007/s00299-024-03237-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s00299-024-03237-4

Keywords

Navigation