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Benzoxazinoids secreted by wheat root weaken the pathogenicity of Fusarium oxysporum f. sp. fabae by inhibiting linoleic acid and nucleotide metabolisms

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Abstract

Key message

The regulatory action of BXs secreted by wheat on the pathogenicity of FOF causing Fusarium wilt in faba bean were analyzed. DIMBOA and MBOA weakened the pathogenicity of FOF.

Abstract

A large number of pathogenic bacteria in continuous cropping soil infect faba bean plants, leading to the occurrence of wilt disease, which restricts their production. Faba bean–wheat intercropping is often used to alleviate this disease. This study investigates the effect of benzoxazinoids (BXs) secreted by wheat root on the pathogenicity of Fusarium oxysporum f. sp. Fabae (FOF) and underlying molecular mechanisms. The effects of DIMBOA(2,4-dihydroxy-7-methoxy-1,4-benzoxazine-4-one) and MBOA(6-methoxybenzoxazolin-2-one) on the activity of cell-wall-degrading enzymes in FOF(cellulase, pectinase, amylase, and protease), FOF Toxin (fusaric acid, FA) content were investigated through indoor culture experiments. The effect of BXs on the metabolic level of FOF was analyzed by metabonomics to explore the ecological function of benzoxazines intercropping control of Fusarium wilt in faba bean. The results show that the Exogenous addition of DIMBOA and MBOA decreased the activity of plant-cell-wall-degrading enzymes and fusaric acid content and significantly weakened the pathogenicity of FOF. DIMBOA and MBOA significantly inhibited the pathogenicity of FOF, and metabolome analysis showed that DIMBOA and MBOA reduced the pathogenicity of FOF by down-regulating related pathways such as nucleotide metabolism and linoleic acid metabolism, thus effectively controlling the occurrence of Fusarium wilt in faba bean.

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Acknowledgements

The authors would like to thank all the reviewers who participated in the review, as well as MJEditor (www.mjeditor.com) for providing English editing services during the preparation of this manuscript.

Funding

This work was supported by the National Natural Science Foundation of China (32260802).

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Author notes

  1. Guanglei Ma and Bijie Hu contributed equally to this work.

Authors and Affiliations

  1. College of Resources and Environment, Yunnan Agricultural University, Kunming, China

    Guanglei Ma, Bijie Hu, Siyin Yang, Zixuan Cen, Yiran Zheng & Yan Dong

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  1. Guanglei Ma
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  2. Bijie Hu
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  3. Siyin Yang
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  4. Zixuan Cen
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  6. Yan Dong
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Contributions

GM conceived the original screening and research plans, finished writing this thesis. YD supervised the experiments agreed to serve as the author responsible for contact and ensures communication. BH provided technical assistance to GM. SY and ZC designed the experiments. GM and YZ analyzed the data. All authors contributed to the article and approved the submitted version.

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Correspondence to Yan Dong.

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The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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Communicated by Zhanyuan Jon Zhang.

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Ma, G., Hu, B., Yang, S. et al. Benzoxazinoids secreted by wheat root weaken the pathogenicity of Fusarium oxysporum f. sp. fabae by inhibiting linoleic acid and nucleotide metabolisms. Plant Cell Rep 43, 109 (2024). https://doi.org/10.1007/s00299-024-03188-w

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