Abstract
Key message
The first-time generation of hexaploid triticale plants harbouring variable panels of novel mutations in gene families involved in starch biosynthesis has been achieved by the subgenome-independent multiplexed CRISPR/Cas9-mediated editing.
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The data supporting the findings of this study are available within the paper and the supplementary materials published online; further inquiries can be directed to the corresponding author.
References
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Funding
This work was supported by KGC-ARRIAB, agreement #075-15-2019-1667.
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DM, VT, MD, GK and SD designed the study, interpreted the results and wrote the article; VT identified genetic targets and constructed the vector. DM-generated transgenic plants. DM, AP and VA performed molecular characterization and seed analysis. PK, MB, MS, AE and MD, designed and conducted HRFA/NGS experiments, and analysed the sequencing data. All authors read and approved the manuscript.
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Communicated by Neal Stewart.
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Miroshnichenko, D., Timerbaev, V., Divashuk, M. et al. CRISPR/Cas9-mediated мultiplexed multi-allelic mutagenesis of genes located on A, B and R subgenomes of hexaploid triticale. Plant Cell Rep 43, 59 (2024). https://doi.org/10.1007/s00299-023-03139-x
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DOI: https://doi.org/10.1007/s00299-023-03139-x