Abstract
Key message
NtTAS14-like1 enhances osmotic tolerance through coordinately activating the expression of osmotic- and ABA-related genes.
Abstract
Osmotic stress is one of the most important limiting factors for tobacco (Nicotiana tabacum) growth and development. Dehydrin proteins are widely involved in plant adaptation to osmotic stress, but few of these proteins have been functionally characterized in tobacco. Here, to identify genes required for osmotic stress response in tobacco, an encoding dehydrin protein gene NtTAS14-like1 was isolated based on RNA sequence data. The expression of NtTAS14-like1 was obviously induced by mannitol and abscisic acid (ABA) treatments. Knock down of NtTAS14-like1 expression reduced osmotic tolerance, while overexpression of NtTAS14-like1 conferred tolerance to osmotic stress in transgenic tobacco plants, as determined by physiological analysis of the relative electrolyte leakage and malonaldehyde accumulation. Further expression analysis by quantitative real-time PCR indicated that NtTAS14-like1 participates in osmotic stress response possibly through coordinately activating osmotic- and ABA-related genes expression, such as late embryogenesis abundant (NtLEA5), early responsive to dehydration 10C (NtERD10C), calcium-dependent protein kinase 2 (NtCDPK2), ABA-responsive element-binding protein (NtAREB), ABA-responsive element-binding factor 1 (NtABF1), dehydration-responsive element-binding genes (NtDREB2A), xanthoxin dehydrogenase/reductase (NtABA2), ABA-aldehyde oxidase 3 (NtAAO3), 9-cis-epoxycarotenoid dioxygenase (NtNCED3). Together, this study will facilitate to improve our understandings of molecular and functional properties of plant TAS14 proteins and to improve genetic evidence on the involvement of the NtTAS14-like1 in osmotic stress response of tobacco.
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All data supporting the findings of this study are available within this paper.
Abbreviations
- ABA:
-
Abscisic acid
- DREB:
-
Dehydration-responsive element-binding genes
- NCED:
-
9-cis-Epoxycarotenoid dioxygenase
- ABA2:
-
Xanthoxin dehydrogenase/reductase
- AAO3:
-
ABA-aldehyde oxidase 3
- DHN proteins:
-
Dehydrins
- LEA:
-
Late embryogenesis abundant
- VIGS:
-
Virus-induced gene silencing
- EL:
-
Relative electrolyte leakage
- MDA:
-
Malonaldehyde
- DS:
-
Drought stress
- AREB:
-
ABA-responsive element-binding protein
- ERD10C:
-
Early responsive to dehydration 10C
- WT:
-
Wild type
- ABF1:
-
ABA-responsive element-binding factor 1
- qRT-PCR:
-
Quantitative real-time PCR
- RNA-seq:
-
RNA sequence
- CDPK2:
-
Calcium-dependent protein kinase 2
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Funding
This work was supported by the Science and Technology Program of Hunan Provincial Tobacco Corporation (Grant Nos: HN2021KJ02, HN2021KJ04, 19-23Aa01) and the National Natural Science Foundation of China (Grant No 32201459).
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XH and RH conceived and designed the experiments. XH, ZH and YL performed most of the experiments. JY, SS, XL, and PY performed some of the experiments. XH and ZH wrote the manuscript. RH, YL, CX, and CL gave advices and revised the manuscript. All authors read and approved the manuscript.
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Hu, Z., Li, Y., Yang, J. et al. The positive impact of the NtTAS14-like1 gene on osmotic stress response in Nicotiana tabacum. Plant Cell Rep 43, 25 (2024). https://doi.org/10.1007/s00299-023-03118-2
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DOI: https://doi.org/10.1007/s00299-023-03118-2