Abstract
Key message
Autophagy receptor OsNBR1 modulates salt stress tolerance by affecting ROS accumulation in rice.
Abstract
The NBR1 (next to BRCA1 gene 1), as important selective receptors, whose functions have been reported in animals and plants. Although the function of NBR1 responses to abiotic stress has mostly been investigated in Arabidopsis thaliana, the role of NBR1 under salt stress conditions remains unclear in rice (Oryza sativa). In this study, by screening the previously generated activation-tagged line, we identified a mutant, activation tagging 10 (AC10), which exhibited salt stress-sensitive phenotypes. TAIL-PCR (thermal asymmetric interlaced PCR) showed that the AC10 line carried a loss-of-function mutation in the OsNBR1 gene. OsNBR1 was found to be a positive regulator of salt stress tolerance and was localized in aggregates. A loss-of-function mutation in OsNBR1 increased salt stress sensitivity, whereas overexpression of OsNBR1 enhanced salt stress resistance. The osnbr1 mutants showed higher ROS (reactive oxygen species) production, whereas the OsNBR1 overexpression (OsNBR1OE) lines showed lower ROS production, than Kitaake plants under normal and salt stress conditions. Furthermore, RNA-seq analysis revealed that expression of OsRBOH9 (respiratory burst oxidase homologue) was increased in osnbr1 mutants, resulting in increased ROS accumulation in osnbr1 mutants. Together our results established that OsNBR1 responds to salt stress by influencing accumulation of ROS rather than by regulating transport of Na+ and K+ in rice.
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The Sequence Read Archive (SRA) database at the National Center for Biotechnology Information (NCBI) has received the RNA-seq data generated in this investigation and has been assigned the accession number PRJNA1022517. This published article and its supplemental information files contain all of the data created or analyzed during this study.
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This work was supported by the National Natural Science Foundation of China (32272027, 32102235) and Natural Science Foundation of Jilin Province (20230101259JC).
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Z-YX devised the project. Z-YX and YL supervised the project. AM performed the most physiological analyses and wrote the manuscript. NN performed the most of biochemical assays and some physiological analyses. YS performed molecular cloning and some cell biological analyses. JW performed RNA-seq and data analysis. PG, JY, WL, GZ, and DZ performed some biochemical assays. D-JY provided helpful suggestions for improving the manuscript.
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Ma, A., Nan, N., Shi, Y. et al. Autophagy receptor OsNBR1 modulates salt stress tolerance in rice. Plant Cell Rep 43, 17 (2024). https://doi.org/10.1007/s00299-023-03111-9
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DOI: https://doi.org/10.1007/s00299-023-03111-9