Abstract
Key message
OsMKK1, a MAPK gene, positively regulates rice Xa21-mediated resistance response and also plays roles in normal growth and development process of rice.
Abstract
The mitogen-activated protein kinase (MAPK) cascade was highly conserved among eukaryotes, which played crucial roles in plant responses to pathogen infection. Bacterial blight is the most devastating bacterial disease. Xa21 confers broad-spectrum resistance to Xanthomonas oryzae pv. Oryzae (Xoo). This study identified that the transcription level of OsMKK1 was up-regulated in resistant response against Xoo, thus overexpression (OsMKK1-OX) and RNA interference (OsMKK1-RNAi) transgenic rice lines under the background of Xa21 was constructed. Compared with recipient control plants 4021, the OsMKK1-OX lines significantly enhanced disease resistance to Xoo, on the contrary, the resistance of OsMKK1-RNAi lines was weakened, demonstrated that OsMKK1 played a positive role in Xa21-mediated disease resistance pathway. A number of pathogenesis-related proteins, including PR1A, PR2 and PR10A showed enhanced expression in OsMKK1-OX lines, supported that these PR genes may be regulated by OsMKK1 to participate in the defense responses. In addition, the agronomic traits of OsMKK1 transgenic plants were affected. Overall, these results revealed the role of OsMKK1 in Xa21-mediated resistance against Xoo and in the normal growth and development process in rice.
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Funding
The research was supported by Natural Science Foundation of Hebei Province of China (C2022204128), Hebei Key Laboratory of Plant Physiology and Molecular Pathology, Baoding, 071001, China and the Start-up Fund for Introduced Talents of Hebei Agricultural University (YJ201910).
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ZY, ZZ, YG, SC and JL participated in the study design and performed the experiments, collected and analyzed the data. JZ, SD and MY participated in the resources, validation and methodology experiments. ZY, LL and GL wrote and revised the manuscript. All authors read and approved the final manuscript.
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Communicated by Woe-Yeon Kim.
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Yang, Z., Zhu, Z., Guo, Y. et al. OsMKK1 is a novel element that positively regulates the Xa21-mediated resistance response to Xanthomonas oryzae pv. oryzae in rice. Plant Cell Rep 43, 31 (2024). https://doi.org/10.1007/s00299-023-03085-8
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DOI: https://doi.org/10.1007/s00299-023-03085-8