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dCas9-3xSRDX-mediated transcriptional repression in sugarcane

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Abstract

Key message

Targeting dCas9 fused with the 3xSRDX effector to the 5’UTR leads to strong repression of magnesium chelatase in highly polyploid sugarcane.

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Acknowledgements

This research was funded by the DOE Center for Advanced Bioenergy and Bioproducts Innovation (U.S. Department of Energy, Office of Science, Office of Biological and Environmental Research) under award number DE-SC0018420. Any opinions, findings and conclusions, or recommendations expressed in this publication are those of the authors and do not necessarily reflect the views of the U.S. Department of Energy.

Funding

The funding was supported by Biological and Environmental Research, DE-SC0018420, Fredy Altpeter.

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Authors and Affiliations

Authors

Contributions

FA conceived and managed the research project. FA and IH designed the experiments. IH performed the experiments. FA and IH wrote and approved the manuscript.

Corresponding author

Correspondence to Fredy Altpeter.

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The authors declare no conflict of interest.

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Communicated by Neal Stewart.

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Hooghvorst, I., Altpeter, F. dCas9-3xSRDX-mediated transcriptional repression in sugarcane. Plant Cell Rep 42, 1837–1840 (2023). https://doi.org/10.1007/s00299-023-03062-1

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  • DOI: https://doi.org/10.1007/s00299-023-03062-1

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