Abstract
Key message
Targeting dCas9 fused with the 3xSRDX effector to the 5’UTR leads to strong repression of magnesium chelatase in highly polyploid sugarcane.
Similar content being viewed by others
References
Eid A, Mohan C, Sanchez S, Wang D, Altpeter F (2021) Multiallelic, targeted mutagenesis of magnesium chelatase with CRISPR/Cas9 provides a rapidly scorable phenotype in highly polyploid sugarcane. Front Genome Ed 3:654996
Hiratsu K, Matsui K, Koyama T, Ohme-Takagi M (2003) Dominant repression of target genes by chimeric repressors that include the EAR motif, a repression domain, in Arabidopsis. Plant J 34:733–739
Jung JH, Vermerris W, Gallo M, Fedenko JR, Erickson JE, Altpeter F (2013) RNA interference suppression of lignin biosynthesis increases fermentable sugar yields for biofuel production from field-grown sugarcane. Plant Biotechnol J 11:709–716
Kannan B, Jung JH, Moxley GW, Lee S-M, Altpeter F (2018) TALEN-mediated targeted mutagenesis of more than 100 COMT copies/alleles in highly polyploid sugarcane improves saccharification efficiency without compromising biomass yield. Plant Biotechn J 16:856–866
Lowder LG, Zhang D, Baltes NJ, Paul JW III, Tang X, Zheng X, Voytas DF, Hsieh T-F, Zhang Y, Qi Y (2015) A CRISPR/Cas9 toolbox for multiplexed plant genome editing and transcriptional regulation. Plant Physiol 169:971–985
Papenbrock J, Mock H-P, Tanaka R, Kruse E, Grimm B (2000) Role of magnesium chelatase activity in the early steps of the tetrapyrrole biosynthetic pathway. Plant Physiol 122:1161–1170
Piatek A, Ali Z, Baazim H, Li L, Abulfaraj A, Al-Shareef S, Aouida M, Mahfouz MM (2015) RNA-guided transcriptional regulation in planta via synthetic dCas9-based transcription factors. Plant Biotechnol J 13:578–589
Shalem O, Sanjana N, Zhang F (2015) High-throughput functional genomics using CRISPR–Cas9. Nat Rev Genet 16:299–311. https://doi.org/10.1038/nrg3899
Willows RD, Gibson LCD, Kanangara CG, Hunter CN, Von Wettstein D (1996) Three separate proteins constitute the magnesium chelatase of Rhodobacter sphaeroides. Eur J Biochem 235:438–443
Zhou H, Xu L, Li F, Li Y (2022) Transcriptional regulation by CRISPR/dCas9 in common wheat. Gene 807:145919
Acknowledgements
This research was funded by the DOE Center for Advanced Bioenergy and Bioproducts Innovation (U.S. Department of Energy, Office of Science, Office of Biological and Environmental Research) under award number DE-SC0018420. Any opinions, findings and conclusions, or recommendations expressed in this publication are those of the authors and do not necessarily reflect the views of the U.S. Department of Energy.
Funding
The funding was supported by Biological and Environmental Research, DE-SC0018420, Fredy Altpeter.
Author information
Authors and Affiliations
Contributions
FA conceived and managed the research project. FA and IH designed the experiments. IH performed the experiments. FA and IH wrote and approved the manuscript.
Corresponding author
Ethics declarations
Conflict of interest
The authors declare no conflict of interest.
Additional information
Communicated by Neal Stewart.
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.
About this article
Cite this article
Hooghvorst, I., Altpeter, F. dCas9-3xSRDX-mediated transcriptional repression in sugarcane. Plant Cell Rep 42, 1837–1840 (2023). https://doi.org/10.1007/s00299-023-03062-1
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00299-023-03062-1