Abstract
Key message
This study provides important information on the genetic basis of GCaC in wheat, thus contributing to breeding efforts to improve the nutrient quality of wheat.
Abstract
Calcium (Ca) plays important roles in the human body. Wheat grain provides the main diet for billions of people worldwide but is low in Ca content. Here, grain Ca content (GCaC) of 471 wheat accessions was determined in four field environments. A genome-wide association study (GWAS) was performed to reveal the genetic basis of GCaC using the phenotypic data form four environments and a wheat 660 K single nucleotide polymorphism (SNP) array. Twelve quantitative trait locus (QTLs) for GCaC were identified on chromosomes 1A, 1D, 2A, 3B, 6A, 6D, 7A, and 7D, which was significant in at least two environments. Haplotype analysis revealed that the phenotypic difference between the haplotypes of TraesCS6D01G399100 was significant (P ≤ 0.05) across four environments, suggesting it as an important candidate gene for GCaC. This research enhances our understanding of the genetic architecture of GCaC for further improving the nutrient quality of wheat.
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Data availability
The data for the study is available upon reasonable request from the corresponding author.
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Acknowledgements
The authors want to thank Dr. Zhang Chenyang in School of Chemistry & Materials Engineering, Xinxiang University for assistance in ICP analysis. This study was supported by the National Key Research and Development Program of China (2018YFD0300703-2 and 2017YFD0301101); the Open Project Program (CSBAA2020001) of State Key Laboratory of Crop Stress Biology for Arid Areas, NWAFU, Yangling, Shaanxi, 712100, China.
Funding
This study was supported by the National Key Research and Development Program of China (2018YFD0300703-2 and 2017YFD0301101); the Open Project Program (CSBAA2020001) of State Key Laboratory of Crop Stress Biology for Arid Areas, NWAFU, Yangling, Shaanxi, 712100, China.
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JM: Conceptualization, methodology, software, validation, formal analysis, investigation, data curation, writing—original draft preparation. JR: validation, investigation, writing—original draft preparation. XY: validation, investigation. MY: methodology, formal analysis, investigation, data curation, writing—original draft preparation. DZ: investigation. CL: investigation. QZ: methodology, software, data curation. JW: methodology. DH: Conceptualization, methodology, software, resources, writing—review and editing. LJ: Conceptualization, writing—review and editing, funding acquisition. All authors read and approved the final manuscript.
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Ma, J., Ren, J., Yuan, X. et al. Genome-wide association study reveals the genetic variation and candidate gene for grain calcium content in bread wheat. Plant Cell Rep 42, 1379–1390 (2023). https://doi.org/10.1007/s00299-023-03036-3
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DOI: https://doi.org/10.1007/s00299-023-03036-3