Abstract
Key message
IiSVP of Isatis indigotica was cloned and its expression pattern was analyzed. Ectopic expression of IiSVP in Arabidopsis could delay the flowering time and reduce the size of the floral organs.
Abstract
SVP (SHORT VEGETATIVE PHASE) can negatively regulate the flowering time of Arabidopsis. In the present work, the cDNA of IiSVP, an orthologous gene of AtSVP in I. indigotica, was cloned. IiSVP was highly expressed in rosette leaves, inflorescences and petals, but weakly expressed in sepals, pistils and young silicles. The results of subcellular localization showed that IiSVP was localized in nucleus. Bioinformatics analysis indicated that this protein was a MADS-box transcription factor. Constitutive expression of IiSVP in Arabidopsis thaliana resulted in decrease of the number of petals and stamens, and curly sepals were formed. In IiSVP transgenic Arabidopsis plants, obvious phenotypic variations in flowers could be observed, especially the size of the floral organs. In comparison with the wild-type plants, the size of petals, stamens and pistil in IiSVP transgenic Arabidopsis plants was decreased significantly. In some transgenic plants, the petals were wrapped by the sepals. Yeast two-hybrid experiments showed that IiSVP could form higher-order complexes with other MADS proteins, including IiSEP1, IiSEP3, IiAP1 and IiSEP4, but could not interact with IiSEP2. In this work, it was proved that the flowering process and the floral development in Arabidopsis could be affected by IiSVP from I. indigotica Fortune.
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Acknowledgements
This work was supported by the National Natural Science Foundation of China (31300223, 30870194, J1210063), and the Major Project of Basic Research Program of Natural Sciences of Shaanxi Province (2021JZ-41).
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QM, ZQP and ZQX conceived and designed the study; QM, XFH, HC, XMT and ZQP performed the experiments and analyzed the data; ZQX wrote the paper.
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Meng, Q., Hou, XF., Cheng, H. et al. IiSVP of Isatis indigotica can reduce the size and repress the development of floral organs. Plant Cell Rep 42, 561–574 (2023). https://doi.org/10.1007/s00299-023-02977-z
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DOI: https://doi.org/10.1007/s00299-023-02977-z