Abstract
Key message
OsVP1 and Sdr4 play an important role in regulating seed dormancy that involved in multiple metabolism and regulatory pathways.
Abstract
Seed dormancy and germination are critical agricultural traits influencing rice grain yield. Although there are some genes have identified previously, the comprehensive understanding based on transcriptome is still deficient. In this study, we generated mutants of two representative regulators of seed germination, Oryza sativa Viviparous1 (OsVP1) and Seed dormancy 4 (Sdr4), by CRISPR/Cas9 approach and named them cr-osvp1 and cr-sdr4. The weakened dormancy of mutants indicated that the functions of OsVP1 and Sdr4 are required for normal early seed dormancy. There were 4157 and 8285 differentially expressed genes (DEGs) were identified in cr-osvp1 vs. NIP and cr-sdr4 vs. NIP groups, respectively, with a large number of overlapped DEGs between two groups. The gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis of common DEGs in two groups showed that genes related to carbohydrate metabolic, nucleoside metabolic, amylase activity and plant hormone signal transduction were involved in the dormancy regulation. These results suggest that OsVP1 and Sdr4 play an important role in regulating seed dormancy by multiple metabolism and regulatory pathways. The systematic analysis of the transcriptional level changes provides theoretical basis for the research of seed dormancy and germination in rice.
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The datasets generated and/or analyzed in the course of this study are available from the corresponding authors on reasonable request.
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This research was financially supported by the National Natural Science Foundation of China (32072528, 31872051), and Shenzhen Technology Research & Development (JCYJ20200109150713553).
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WQC designed and performed most of the experiments; JL provided instructions for the experiments; JLX conceived the project and supervised the experiments; ZXL performed the transcriptome data analysis; WQC analyzed the data and wrote the manuscript; DPL, KC and JLX revised the article.
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Communicated by Kinya Toriyama.
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Supplementary file1 Table S1. Significant GO terms of DEGs in the biological process category for cr-osvp1 vs. NIP. Table S2. Significant GO terms of DEGs in the cellular component category for cr-osvp1 vs. NIP. Table S3. Significant GO terms of DEGs in molecular function category for cr-osvp1 vs. NIP. Table S4. Significant GO terms of DEGs in the biological process category for cr-sdr4 vs. NIP. Table S5. Significant GO terms of DEGs in the cellular component category for cr-sdr4 vs. NIP. Table S6. Significant GO terms of DEGs in molecular function category for cr-sdr4 vs. NIP. Table S7. Primers of qRT-PCR. Fig. S1 Amino acid sequence alignment. (PDF 317 KB)
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Chen, WQ., Liu, DP., Li, ZX. et al. Transcriptome analysis of knockout mutants of rice seed dormancy gene OsVP1 and Sdr4. Plant Cell Rep 42, 309–319 (2023). https://doi.org/10.1007/s00299-022-02958-8
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DOI: https://doi.org/10.1007/s00299-022-02958-8