Abstract
Key message
Expression of the VaRPP13 in Arabidopsis and tobacco enhanced resistance to oomycete pathogens, and this enhancement is closely related to the activation of salicylic acid (SA) signaling pathway.
Abstract
Resistance (R) genes, which usually contain a nucleotide-binding site and a leucine-rich repeat (NBS-LRR) domain, play crucial roles in disease resistance. In this study, we cloned a CC-NBS-LRR gene VaRPP13 from Vitis amurensis ‘Shuang Hong’ grapevine, and investigated its function on disease resistance. VaRPP13 expression was induced by Plasmopara viticola, an oomycetes pathogen causing downy mildew disease in grapevine. Heterologous expression VaRPP13 could also enhance resistance to Hyaloperonospora arabidopsidis in Arabidopsis thaliana and Phytophthora capsici in Nicotiana benthamiana, both oomycete pathogens. Further study indicated that VaRPP13 could enhance the expression of genes in SA signal pathway, while exogenous SA could also induce the expression of VaRPP13. In conclusion, our studies demonstrated that VaRPP13 contributes to a broad-spectrum resistance to oomycetes via activating SA signaling pathway.
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Abbreviations
- CC:
-
Coiled coil
- CNL:
-
CC-NBS-LRR
- DAB:
-
Diaminobenzidine
- EDS1 :
-
Enhanced disease susceptibility 1
- ETI:
-
Effector-triggered immunity
- H. a. Noco2:
-
Hyaloperonospora arabidopsidis Noco2
- Hpi:
-
Hours post inoculation
- HR:
-
Hypersensitive response
- JA:
-
Jasmonic acid
- LRR:
-
Leucine-rich repeat
- MAPK:
-
Mitogen-activated protein kinase
- NBS:
-
Nucleotide-binding site
- NBT:
-
Nitro blue tetrazolium
- NPR1 :
-
Nonexpressor of pathogenesis-related gene
- PAMPs:
-
Pathogen-associated molecular patterns
- PCR:
-
Polymerase chain reaction
- PR1 :
-
Pathogenesis-related gene 1
- PR2 :
-
Pathogenesis-related gene 2
- PRRs:
-
Pattern recognition receptors
- PstDC3000:
-
Pseudomonas syringae Pv. tomato DC3000
- PTI:
-
PAMP-Triggered immunity
- Pv:
-
Plasmopara viticola
- qRT-PCR:
-
Quantitative real-time PCR
- R :
-
Genes resistance genes
- RNL:
-
RPW8-NBS-LRR
- ROS:
-
Reactive oxygen species
- RPW8:
-
Resistance to powdery mildew8
- SA:
-
Salicylic acid
- TIR:
-
Toll/interleukin-1 receptor
- TNL:
-
TIR-NBS-LRR
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Acknowledgements
This work was supported by National Key Research & Development Program of China (2018YFD1000301) and Shanghai Municipal Agricultural Commission (grant no. 2021-02-08-00-12-F00751).
Funding
This study was funded by a grant (2018YFD1000301) of the National Key Research & Development Program funded by Ministry of Science and Technology of the People's Republic of China (PRC), and a grant (2021-02-08-00-12-F00751) of the Agriculture Applied Technology Development Program funded by Shanghai Municipal Agricultural Commission, PRC.
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Chen, Y., Wu, W., Yang, B. et al. Grapevine VaRPP13 protein enhances oomycetes resistance by activating SA signal pathway. Plant Cell Rep 41, 2341–2350 (2022). https://doi.org/10.1007/s00299-022-02924-4
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DOI: https://doi.org/10.1007/s00299-022-02924-4