Abstract
Key message
Tomato DWARF14 regulates the development of roots, shoot branches and leaves, and also plays a role in photosynthetic pigment accumulation and photosynthetic capacity.
Abstract
Strigolactones (SLs) are a novel class of plant hormones. DWARF14 (D14) is the only SL receptor identified to date, but it is not functionally analyzed in tomato (Solanum lycopersicum). In the present study, we identified the potential SL receptor in tomato by bioinformatic analysis, which was designated as SlD14. SlD14 was expressed in roots, stems, flowers and developing fruits, with the highest expression level in leaves. sld14 mutant plants produced by the CRISPR/Cas9 system displayed reduced plant height and root biomass, increased shoot branching and altered leaf shape comparing with WT plants. The cytokinin biosynthetic gene ISOPENTENYLTRANSFERASE 3 (SlIPT3), auxin biosynthetic genes FLOOZY (SlFZY) and TRYPTOPHAN AMINOTRANSFERASE RELATED 1 (SlTAR1) and several auxin transport genes SlPINs, which are involved in branch formation, showed higher expression levels in the sld14 plant stem. In addition, sld14 plants exhibited light-green leaves, reduced chlorophyll and carotenoid contents, abnormal chloroplast structure and reduced photosynthetic capacity. Transcriptomic analysis showed that the transcript levels of six chlorophyll biosynthetic genes, three carotenoid biosynthetic genes and numerous chlorophyll a/b-binding protein genes were decreased in sld14 plants. These results suggest that tomato SL receptor gene SlD14 not only regulates the development of roots, shoot branches and leaves, but also plays a role in regulating photosynthetic pigment accumulation and photosynthetic capacity.
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The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.
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Acknowledgements
This work was supported by the Jiangsu Seed Industry Revitalization Project [JBGS(2021)015], National Key Research and Development Program of China (2020YFE0202900), Natural Science Foundation of Jiangsu Province (BK20221511), Jiangsu Agricultural Science and Technology Innovation Fund [CX(20)3105], and Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).
Funding
The work of Han Wu was supported by Jiangsu Seed Industry Revitalization Project, JBGS(2021)015, National Key Research and Development Program of China, 2020YFE0202900, Natural Science Foundation of Jiangsu Province, BK20221511, and Jiangsu Agricultural Science and Technology Independent Innovation Fund, CX(20)3105.
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HW and YL conceived and designed research. ZL, YP, CZ, DX, WM, and HC conducted experiments. ZL, YP, and HW analyzed data. ZL, HW, and YL wrote the manuscript. All authors read and approved the manuscript.
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Li, Z., Pi, Y., Zhai, C. et al. The strigolactone receptor SlDWARF14 plays a role in photosynthetic pigment accumulation and photosynthesis in tomato. Plant Cell Rep 41, 2089–2105 (2022). https://doi.org/10.1007/s00299-022-02908-4
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DOI: https://doi.org/10.1007/s00299-022-02908-4