Abstract
Key message
Forty PaCRKs have been identified from sweet cherry and overexpression PaCRK1 in sweet cherry enhances its resistance to salt stress.
Abstract
Cysteine-rich receptor-like kinases (CRKs), a large subgroup of the receptor-like kinases, play an important role in plant development and stress response. However, knowledge about CRKs and its function against adverse environmental stresses in sweet cherry were lacking. In this study, 40 PaCRKs were identified from sweet cherry (Prunus avium) genome database. Phylogenetic analysis indicated that PaCRKs could be classified into six subgroups. Transcriptome analysis showed that the expression levels of most PaCRKs were changed under external environmental stresses. Functional study showed that PaCRK1 overexpression could enhance Arabidopsis and sweet cherry tolerance to salt stress. Moreover, biochemical analysis showed that PaCRK1 increased salt tolerance of sweet cherry by regulating the expression of antioxidation-related genes and their enzyme activities. This study provides a comprehensive understanding of PaCRKs in sweet cherry and elucidates the potential role of PaCRKs in response to various environmental stimuli.
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Data availability
The RNA-Seq data has been deposited to the SRA of NCBI (https://www.ncbi.nlm.nih.gov/biosample) with the data set Submission ID SUB11195318 and BioProject ID PRJNA816669.
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Acknowledgements
This study was supported by the Improved Seed Project of Shandong Province (2020LZGC008), the National Natural Science Foundation of China (32071788 and 32171810), the Natural Science Foundation of Shandong Province (ZR2021MC102, ZR2020MC153 and ZR2020MC135).
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Zhao, X., Qu, D., Wang, L. et al. Genome-wide identification of cysteine-rich receptor-like kinases in sweet cherry reveals that PaCRK1 enhances sweet cherry resistance to salt stress. Plant Cell Rep 41, 2037–2088 (2022). https://doi.org/10.1007/s00299-022-02907-5
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DOI: https://doi.org/10.1007/s00299-022-02907-5