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MTO1-RESPONDING DOWN 1 (MRD1) is a transcriptional target of OZF1 for promoting salicylic acid-mediated defense in Arabidopsis

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Abstract

Key message

OZF1 promotes the transcription of MRD1, which is essential for SA-mediated defense against virulent and avirulent bacterial pathogens in Arabidopsis.

Abstract

Salicylic acid (SA) is critical for defense against biotrophic pathogens. A trans-activator protein NPR1 plays significant roles in SA-signaling. However, evidences suggest the existence of NPR1-independent pathways for SA signaling in plants. Previously, we reported Arabidopsis OXIDATION-RELATED ZN-FINGER PROTEIN1 (OZF1) as a positive regulator of NPR1-independent SA-signaling. However, the mechanism or components of OZF1-mediated SA signaling was not known. Through the analysis of differentially expressing genes, we report the identification of MTO1-RESPONDING DOWN 1 (MRD1) as a transcriptional target of OZF1. Expressions of MRD1 and its overlapping gene in Arabidopsis genome, HEI10 increase upon pathogen inoculation in an OZF1-dependent manner. Their mutants are susceptible to both virulent and avirulent bacterial pathogens and show compromised SA-mediated immunity. Overexpression of MRD1 but not the HEI10 rescues the loss-of-resistance phenotype of the ozf1 mutant. OZF1 physically associates at the MRD1 promoter area upon pathogen inoculation. Results altogether support that MRD1 is a transcriptional target of OZF1 for promoting SA-mediated defense in Arabidopsis.

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Acknowledgements

Authors acknowledge ABRC, Ohio State University, USA, for mutant seeds, Genotypic Technology, Bangalore, India for microarray analysis, and DST-FISTII of JNU for infrastructural support. A. Singh, and N. Singh Received fellowship from UGC, A. Sharma received fellowship from CSIR.

Funding

This work was primarily supported by the CSIR Grant (38(1515)/21/EMR-II) to AKN.

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Authors and Affiliations

  1. 415, School of Life Sciences, Jawaharlal Nehru University, New Delhi, 110067, India

    Anupriya Singh, Akash Sharma, Nidhi Singh & Ashis Kumar Nandi

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  1. Anupriya Singh
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  2. Akash Sharma
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  3. Nidhi Singh
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  4. Ashis Kumar Nandi
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Contributions

AKN conceptualized and designed the experiment. AS, NS, and AS performed the experiments. AS wrote the manuscript, which was further modified by AKN and approved by all authors.

Corresponding author

Correspondence to Ashis Kumar Nandi.

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The authors have no conflicts of interest to declare.

Additional information

Communicated by Prakash P. Kumar.

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Supplementary Information

Below is the link to the electronic supplementary material.

Supplementary file1 (XLSX 90 KB) Supplementary dataset-S1-Genes downregulated in ozf1 mutant

Supplementary file2 (XLSX 82 KB) Supplementary dataset-S2-Genes upregulated in ozf1 mutant

299_2022_2861_MOESM3_ESM.pdf

Supplementary file3 (PDF 6251 KB) Fig. S1: Venn diagram showing the number of the differentially expressed gene after Psm inoculation. Fig. S2: Venn diagram showing the number of differentially expressed genes after PstAvrRpt2 inoculation . Fig. S3: Relative abundance of mRNA of selected DEGs between WT and ozf1 mutants after Psm inoculation. Fig. S4. Relative abundance of mRNA of selected DEGs between WT and ozf1 mutants after PstAvrRpt2 inoculation. Fig. S5 pMRD1:GUS, pHEI10:GUS expression in WT, ozf1 and OZF1-Oex plants after SA or mock treatment. Fig. S6. Relative position of At1g53480(MRD1) and At1g54390(HEI10) genes in the Arabidopsis genome with the position of T-DNA insertions in the mutant lines. Fig. S7. Confirmation of T-DNA insertion lines and plant morphology. Fig. S8. RT-PCR for MRD1 and HEI10 expression in transgenic plants. Supplementary Table S1: List of genes selected for further validation of their expression pattern by qPCR. Supplementary Table S2 : List of primers used in the study

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Singh, A., Sharma, A., Singh, N. et al. MTO1-RESPONDING DOWN 1 (MRD1) is a transcriptional target of OZF1 for promoting salicylic acid-mediated defense in Arabidopsis. Plant Cell Rep 41, 1319–1328 (2022). https://doi.org/10.1007/s00299-022-02861-2

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  • DOI: https://doi.org/10.1007/s00299-022-02861-2

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