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Generation of a multi-herbicide-tolerant alfalfa by using base editing

Abstract

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We present the first report on base editing in alfalfa. Specifically, we showed edited alfalfa with tolerance to both sulfonylurea- and imidazolinone-type herbicides.

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Acknowledgements

This work was supported by grants PICT 2017#0484, FVT 2017#39 and PICT-STARTUP 2020 #00004 provided to GS and by PE I115 (Biotechnology Program of INTA, Argentina). EB has a graduate student fellowship from CONICET, Argentina. MS was supported by fellowships from CONICET and IUBMB.

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Contributions

Conceived and designed the experiments: MS and GS. Performed the experiments: EB, CG, MS, HT, RF, CP, NA and GS. Analyzed the data: EB, CG, MS, HT, EB, NA and GS. Wrote the paper: GS. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Gabriela Soto.

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The authors declare that they have no conflict of interest.

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Communicated by Neal Stewart.

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Bottero, E., Gómez, C., Stritzler, M. et al. Generation of a multi-herbicide-tolerant alfalfa by using base editing. Plant Cell Rep 41, 493–495 (2022). https://doi.org/10.1007/s00299-021-02827-w

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  • DOI: https://doi.org/10.1007/s00299-021-02827-w

Keywords

  • Alfalfa
  • Base editing
  • CRISPR/Cas9
  • ALS
  • Herbicides