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Engineering the AEG1 promoter from cotton to develop male sterile lines

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Abstract

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A promoter expressing in anther and roots is made anther specific. The modified promoter is used to drive barnase gene and develop male sterile lines.

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References

  • Ali S, Kim WC (2019) A fruitful decade using synthetic promoters in the improvement of transgenic plants. Front Plant Sci 10:1433

    Article  Google Scholar 

  • Elmayan T, Tepfer M (1995) Evaluation in tobacco of the organ specificity and strength of the rolD promoter, domain A of the 35S promoter and the 35S2 promoter. Transgenic Res 4:388–396

    Article  CAS  Google Scholar 

  • Jagannath A, Bandyopadhyay P, Arumugam N, Gupta V, Burma PK, Pental D (2001) The use of a Spacer DNA fragment insulates the tissue-specific expression of a cytotoxic gene (barnase) and allow high-frequency generation of transgenic male sterile lines in Brassica juncea L. Mol Breeding 8:11–23

    Article  CAS  Google Scholar 

  • Jagannath A, Arumugam N, Gupta V, Pradhan A, Burma P, Pental D (2002) Development of transgenic barstar lines and identification of a male sterile (barnase)/ restorer (barstar) combination for heterosis breeding in Indian oilseed mustard (Brassica juncea). Curr Sci 82(1):46–52

    CAS  Google Scholar 

  • Mariani C, Gossele V, Beuckeleer MD, Block MD, Goldberg RB, Greef WD, Leemans J (1992) A chimeric ribonuclease-inhibitor gene restores fertility to male sterile plants. Nature 357:384–387

    Article  CAS  Google Scholar 

  • Paritosh K, Singh AK, Mehrotra AK, Pental D, Burma PK (2018) Identification and characterization of the promoter of a gene expressing mainly in the tapetum tissue of cotton (Gossypium hirsutum L.). Plant Biotechnol Rep 12:377–388

    Article  Google Scholar 

  • Sharma PA, Verma N, Burma PK (2018) Analysis of activity driven by upstream regulatory modules (URM) of tapetum specific genes TA29 and A9 at ectopic locations in tobacco transgenics. J Plant Biochem Biotechnol 27:443–452

    Article  CAS  Google Scholar 

  • Vijaybhaskar V, Subbiah V, Kaur J, Vijayakumari P, Siddiqi I (2008) Identification of a root-specific glycosyltransferase from Arabidopsis and charcaterization of its promoter. J Biosci 33(2):185–193

    Article  CAS  Google Scholar 

  • Wang XL, Li XB (2009) The GhACS1 gene encodes an acyl-CoA synthetase which is essential for normal microsporogenesis in early anther development of cotton. Plant J 57(3):473–486

    Article  CAS  Google Scholar 

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Acknowledgements

The work was supported by R&D projects of University of Delhi and grant-in-aids to the department from UGC and DST, Govt. of India. KKS and AKM were supported by fellowships from UGC and CSIR, respectively. The authors thank Prof. S C Lakhotia for his edits and Dr. Satish Yadav for help in statistical analysis.

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Contributions

PKB conceptualized the project. PKB, KKS and AKM designed experiments. AKM and KKS carried out experiments. PKB, AKM and KKS analyzed the data. PKB and KKS wrote the manuscript.

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Correspondence to Pradeep Kumar Burma.

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The authors declare no competing interests.

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Communicated by Neal Stewart.

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Soni, K.K., Mehrotra, A.K. & Burma, P.K. Engineering the AEG1 promoter from cotton to develop male sterile lines. Plant Cell Rep 40, 1789–1792 (2021). https://doi.org/10.1007/s00299-021-02704-6

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  • DOI: https://doi.org/10.1007/s00299-021-02704-6