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A triticale tapetal non-specific lipid transfer protein (nsLTP) is translocated to the pollen cell wall

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A Triticeae type III non-specific lipid transfer protein (nsLTP) was shown for the first time to be translocated from the anther tapetum to the pollen cell wall.

Abstract

Two anther-expressed non-specific lipid transfer proteins (nsLTPs) were identified in triticale (× Triticosecale Wittmack). LTPc3a and LTPc3b contain a putative signal peptide sequence and eight cysteine residues in a C-Xn-C-Xn-CC-Xn-CXC-Xn-C-Xn-C pattern. These proteins belong to the type III class of nsLTPs which are expressed exclusively in the inflorescence of angiosperms. The level of LTPc3 transcript in the anther was highest at the tetrad and uninucleate microspore stages, and absent in mature pollen. In situ hybridization showed that LTPc3 was expressed in the tapetal layer of the developing triticale anther. The expression of the LTPc3 protein peaked at the uninucleate microspore stage, but was also found to be associated with the mature pollen. Accordingly, an LTPc3a::GFP translational fusion expressed in transgenic Brachypodium distachyon first showed activity in the tapetum, then in the anther locule, and later on the mature pollen grain. Altogether, these results represent the first detailed characterization of a Triticeae anther-expressed type III nsLTP with possible roles in pollen cell wall formation.

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Author notes

  1. Mohsin Abbas Zaidi

    Present address: Charlottetown Research and Development Centre, Agriculture and Agri-Food Canada, 440 University Avenue, Charlottetown, PE, C1A 4N6, Canada

  2. Stephen J. B. O’Leary

    Present address: Aquatic and Crop Resource Development Research Centre, National Research Council, of Canada, 1411 Oxford Street, Halifax, NS, B3H 3Z1, Canada

  3. Shaobo Wu

    Present address: Institute of Blood Transfusion, Chinese Academy of Medical Sciences and Peking Union Medical College, 26 Huacai Road, Chengdu, 610052, Sichuan, China

  4. Frances Tran

    Present address: Lacombe Research and Development Centre, Agriculture and Agri-Food Canada, 6000 C and E Trail, Lacombe, AB, T4L 1W1, Canada

Authors and Affiliations

  1. Ottawa Research and Development Centre, Agriculture and Agri-Food Canada, 960 Carling Avenue, Ottawa, ON, K1A 0C6, Canada

    Mohsin Abbas Zaidi, Stephen J. B. O’Leary, Christine Gagnon, Denise Chabot, Shaobo Wu, Keith Hubbard, Frances Tran, Dave Sprott, Dhuha Hassan, Steve Gleddie & Laurian S. Robert

  2. Lethbridge Research and Development Centre, Agriculture and Agri-Food Canada, PO Box 3000, Lethbridge, AB, T1J 4B1, Canada

    Tara Vucurevich, Claudia Sheedy & André Laroche

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  1. Mohsin Abbas Zaidi
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Contributions

MAZ contributed to Western and microscopic analyses, performed Brachypodium transformation, transgenic plant and t-DNA mutant analyses, and contributed to writing the manuscript. SJBO isolated the cDNA clones, performed the northern and in situ hybridization analyses. CG carried out the Western blot analyses. DC and KH contributed to the confocal and electron microscopy work. SW and DH generated the protein fusion in E. coli. FT constructed the vector for plant transformation. DS performed the qRT-PCR experiments and RNA-seq analyses. TV and CS generated the anti-LTPc3 antibody. AL, SG and LSR initiated and supervised the research, and contributed to writing the manuscript.

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Correspondence to Laurian S. Robert.

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Zaidi, M.A., O’Leary, S.J.B., Gagnon, C. et al. A triticale tapetal non-specific lipid transfer protein (nsLTP) is translocated to the pollen cell wall. Plant Cell Rep 39, 1185–1197 (2020). https://doi.org/10.1007/s00299-020-02556-6

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