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Hydrogen peroxide alleviates salinity-induced damage through enhancing proline accumulation in wheat seedlings

Plant Cell Reports volume 39, pages 567–575 (2020)Cite this article

Abstract

Key message

NADPH oxidase-mediated H2O2 maintains proline concentration under NaCl stress through regulating its biosynthesis and degradation, conferring salt tolerance to wheat plants.

Abstract

Considerable attention has been paid to the specific role of hydrogen peroxide (H2O2) in plant stress responses. Here, using microscopic, pharmacological and biochemical approaches, we explored H2O2 production and its roles in redox control under salt stress in wheat roots. Exogenous H2O2 pretreatment decreased salt-induced lipid peroxidation, while increased proline content in wheat roots. Salt stress led to a transient increase in NADPH oxidase activity accompanied by accumulation of H2O2 and proline in roots. The elevated proline accumulation in the presence of NaCl was significantly suppressed by diphenyleneiodonium, an inhibitor of NADPH oxidase, and dimethylthiourea, a scavenger of H2O2. The rate-limiting enzyme involved in proline biosynthesis, Δ1-pyrroline-5-carboxylate synthetase (P5CS), was induced by NaCl, whereas the house-keeping enzyme in proline degradation, proline dehydrogenase (ProDH), was inhibited. After 6 h, the activity of P5CS increased by 1.5-fold, whereas ProDH decreased by 13.9%. The levels of these enzymes, however, were restored by NADPH oxidase inhibitor or H2O2 scavenger. After treatment with H2O2, the effects of diphenyleneiodonium and or dimethylthiourea on proline content and activities of P5CS and ProDH were reversed. These results suggested that NADPH oxidase-mediated H2O2 alleviates oxidative damage induced by salt stress through regulating proline biosynthesis and degradation.

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Acknowledgements

This work was financially supported by the National Natural Science Foundation of China (31872167).

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Author notes

  1. Lijuan Liu and Lin Huang contributed equally to this work.

Affiliations

  1. MOE Key Laboratory of Environment Remediation and Ecological Health, College of Natural Resource and Environmental Sciences, Zhejiang University, Hangzhou, 310058, China

    Lijuan Liu, Lin Huang, Xianyong Lin & Chengliang Sun

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  1. Lijuan Liu
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  2. Lin Huang
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  3. Xianyong Lin
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  4. Chengliang Sun
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Contributions

LL, LH and CS designed and performed the experiments; LL analyzed the data; LL, LH, CS and XL wrote the manuscript.

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Correspondence to Chengliang Sun.

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Communicated by Prakash Lakshmanan.

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Liu, L., Huang, L., Lin, X. et al. Hydrogen peroxide alleviates salinity-induced damage through enhancing proline accumulation in wheat seedlings. Plant Cell Rep 39, 567–575 (2020). https://doi.org/10.1007/s00299-020-02513-3

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  • DOI: https://doi.org/10.1007/s00299-020-02513-3

Keywords

  • Salt
  • NADPH oxidase
  • Hydrogen peroxide
  • Proline metabolism
  • Signaling