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The novel protein CSAP accelerates leaf senescence and is negatively regulated by SAUL1 in the dark

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Abstract

Key message

The chloroplast-localized protein CSAP is an ABA-responsive factor and positively regulates dark-induced senescence. This phenomenon is controlled by SAUL1 in Arabidopsis.

Abstract

We report here that CSAP (Chloroplast-localized Senescence-Associated Protein, AT5G39520) functions as a positive regulator of senescence and is controlled by SAUL1 (Senescence Associated E3 Ubiquitin Ligase 1) in Arabidopsis. CSAP transcript level was gradually increased when senescence was progressed. Under dark conditions, the csap mutant showed delayed leaf senescence and reduced chlorophyll breakdown, but overexpression of CSAP accelerated leaf senescence and expressions of chlorophyll catabolic genes were up-regulated compared to the wild-type (WT). NCED3 and AAO3, which are involved in ABA biosynthesis, also showed higher expression in the overexpression lines than the WT. It is known that the CSAP transcript is increased in the saul1 mutant that shows precocious senescence. In our experiments, we confirmed that CSAP interacts with SAUL1 by the yeast two-hybrid and pull-down assays. In addition, we found that SAUL1 decreases the stability of CSAP in the presence of ABA. Taken together, we suggest that CSAP accelerates leaf senescence in the dark and this process is controlled by SAUL1.

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Abbreviations

CSAP:

Chloroplast-localized senescence-associated protein

SAUL1:

Senescence-associated E3 ubiquitin ligase 1

ABA:

Abscisic acid

WT:

Wild type

OX:

Overexpressed

KO:

Knock-out

GUS:

β-glucuronidase

qRT-PCR:

Quantitative reverse transcription-polymerase chain reaction

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Acknowledgements

This work was supported by a grant (PJ01367001) from the Next-Generation BioGreen 21 Program funded by the Rural Development Administration, Republic of Korea. This work was also partially supported by Korea University.

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Author notes

  1. Soo Youn Kim

    Present address: Cloning Department, Bionics, Seoul, 04778, Korea

Authors and Affiliations

  1. Division of Life Sciences, Korea University, Seoul, 02841, Korea

    Won Mi So, Soo Youn Kim, Sujin Hyoung & Jeong Sheop Shin

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  1. Won Mi So
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  2. Soo Youn Kim
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Contributions

WMS and JSS conceived the study. WMS, SYK and JSS designed experiments. WMS, SYK and SH performed experiments. WMS, SYK and JSS wrote the manuscript.

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Correspondence to Jeong Sheop Shin.

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Communicated by Youn-Il Park.

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So, W.M., Kim, S.Y., Hyoung, S. et al. The novel protein CSAP accelerates leaf senescence and is negatively regulated by SAUL1 in the dark. Plant Cell Rep 39, 325–334 (2020). https://doi.org/10.1007/s00299-019-02493-z

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